GeneTex
United States (US)

Estrogen Receptor alpha antibody [6F11]

GTX29269_3610_WB_18121410_420.jpg
GTX29269_3609_IHC-P_18121410_186.jpg
GTX29269_3608_IHC-P_18121410_951.jpg

Cat No. GTX29269

Host Mouse
Clonality Monoclonal
Clone Name 6F11
Isotype IgG1
Application WB, IHC-P, IHC-Fr
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/50
IHC-P 1/40-1/80
IHC-Fr 1/40-1/60

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

IHC-Fr
The use of Zamboni's fixative is recommended for best results.

Not tested in other applications.

Calculated MW

66 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Tissue Culture Supernatant with 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human estrogen receptor (alpha form)

Purification

Tissue Culture Supernatant

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Era Antibody , ESR Antibody , ER Antibody , NR3A1 Antibody , ESRA Antibody , estrogen receptor 1 Antibody , ESR1 Antibody

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES
GTX29269_3610_WB_18121410_420.jpg

GTX29269 WB Image

WB analysis of MCF-7 cell lysate using GTX29269 Estrogen Receptor alpha antibody [6F11].

GTX29269_3609_IHC-P_18121410_186.jpg

GTX29269 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX29269 Estrogen Receptor alpha antibody. Antigen retrieval using citrate buffer at pH6.2.

GTX29269_3608_IHC-P_18121410_951.jpg

GTX29269 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX29269 Estrogen Receptor alpha antibody. Antigen retrieval using citrate buffer at pH6.2.

REFERENCE
Package List Price ($)
$ 379