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Estrogen Receptor alpha antibody [6F11]

Cat. No. GTX75361

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6F11

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human
Package
200 μl ($399)
See all Estrogen Receptor alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/50-1/100
IHC-P 1/40-1/80
IHC-Fr 1/40-1/60

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

IHC-Fr
The use of Zamboni's fixative is recommended for best results.
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

breast carcinoma

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human estrogen receptor (alpha form)

Purification

Tissue Culture Supernatant

Conjugation

Unconjugated

RRID

AB_376743

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1

Cellular Localization

Cytoplasm , Cell membrane , Cell membrane; Single-pass type I membrane protein , Golgi apparatus

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Research Area

REFERENCE

Application Reference

Belcher SM et al. Endocrinology 2009; 150 (3) : 1112-21 Blockade of estrogen receptor signaling inhibits growth and migration of medulloblastoma.
Application : IHC-P
Reactivity : Human

Clone Reference

Col?n-Caraballo M et al. Appl Immunohistochem Mol Morphol 2019; 27 (7):491-500 Human Endometriosis Tissue Microarray Reveals Site-specific Expression of Estrogen Receptors, Progesterone Receptor, and Ki67.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399