*Optimal dilutions/concentrations should be determined by the researcher.
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Not tested in other applications.
This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.
10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Recombinant human Estrogen Receptor alpha protein (aa2-185)
Ab purified from Bioreactor Concentrate by Protein A/G
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Estrogen Receptor 1 , Er , Esr , Esra , Estrr , Era , Nr3A1 , Esr1
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]
GTX34710 IHC-P Image
Formalin-fixed, paraffin-embedded human Breast Invasive Ductal Carcinoma stained with Estrogen Receptor Monoclonal Antibody (ER506).
GTX34710 FACS Image
Flow Cytometry for human ER-alpha on MCF-7 Cells.Black: Cells alone; Green: Isotype Control; Red: PE-labeled ER-alpha Monoclonal Antibody (ER506).