GeneTex
United States (US)

Estrogen Receptor alpha antibody [ER506]

GTX34710_20170712_IHC-P_18121410_948.jpg
GTX34710_GTX34710_20170712_FACS_19021119_572.jpg

Cat No. GTX34710

Host Mouse
Clonality Monoclonal
Clone Name ER506
Isotype IgG1
Application IHC-P, FACS
Reactivity Human
APPLICATION

Application Note

FACS:0.5-1ug/million cells in 0.1ml, IHC-P:.

Calculated MW

66 kDa. ( Note )

Specificity/Sensitivity

This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human Estrogen Receptor alpha protein (aa2-185)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Estrogen Receptor 1,Er,Esr,Esra,Estrr,Era,Nr3A1,Esr1

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES
GTX34710_20170712_IHC-P_18121410_948.jpg

GTX34710 IHC-P Image

Formalin-fixed, paraffin-embedded human Breast Invasive Ductal Carcinoma stained with Estrogen Receptor Monoclonal Antibody (ER506).

GTX34710_GTX34710_20170712_FACS_19021119_572.jpg

GTX34710 FACS Image

Flow Cytometry for human ER-alpha on MCF-7 Cells.Black: Cells alone; Green: Isotype Control; Red: PE-labeled ER-alpha Monoclonal Antibody (ER506).

Package List Price ($)
$ 329