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Estrogen Receptor alpha antibody [ER506]

Anti-Estrogen Receptor alpha antibody [ER506] used in Flow cytometry (FACS). GTX34710
Anti-Estrogen Receptor alpha antibody [ER506] used in Western Blot (WB). GTX34710
Anti-Estrogen Receptor alpha antibody [ER506] used in Protein Array (Protein Array). GTX34710
Anti-Estrogen Receptor alpha antibody [ER506] used in IHC (Paraffin sections) (IHC-P). GTX34710

Cat No. GTX34710

Host

Mouse

Clonality

Monoclonal

Clone Name

ER506

Isotype

IgG1

Application

WB, IHC-P, FACS, Protein Array

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
IHC-P Assay dependent
FACS 1-2μg/10⁶ cells
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Specificity/Sensitivity

This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 2-185) of human Estrogen Receptor alpha protein (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ER , ESR , ESR1 , ESRA , ESTRR , Era , NR3A1 , estrogen receptor 1 , ER alpha , Estrogen Receptor alpha

Cellular Localization

Nucleus,Cytoplasm,Cell membrane,Golgi apparatus

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-Estrogen Receptor alpha antibody [ER506] used in Flow cytometry (FACS). GTX34710

GTX34710 FACS Image

FACS analysis of MCF-7 cells using GTX34710 Estrogen Receptor alpha antibody [ER506].
Black : Blank
Green : Isotype Control
Red : Primary antibody

Anti-Estrogen Receptor alpha antibody [ER506] used in Western Blot (WB). GTX34710

GTX34710 WB Image

WB analysis of MCF-7 cell lysate using GTX34710 Estrogen Receptor alpha antibody [ER506].

Anti-Estrogen Receptor alpha antibody [ER506] used in Protein Array (Protein Array). GTX34710

GTX34710 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Estrogen Receptor, alpha Mouse Monoclonal Antibody (ER506). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-Estrogen Receptor alpha antibody [ER506] used in IHC (Paraffin sections) (IHC-P). GTX34710

GTX34710 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX34710 Estrogen Receptor alpha antibody [ER506].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339