Estrogen Receptor alpha antibody [ESR1/2299R]

Cat. No. GTX02635

Host	Rabbit
Clonality	Monoclonal
Clone Name	ESR1/2299R
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human
Package	100 μg ($529)

See all Estrogen Receptor alpha products

Datasheet

View product citations for antibody GTX02635 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2 μg/ml
IHC-P	1-2 μg/ml
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Observed MW

~67 kDa.

Product Note

This antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human Estrogen Receptor alpha protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1

Cellular Localization

Cytoplasm , Cell membrane , Cell membrane; Single-pass type I membrane protein , Golgi apparatus

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Gene ID: 2099 ESR1
UniProt: P03372 ESR1

Research Area

DATA IMAGES

GTX02635 IHC-P Image

IHC-P analysis of human breast carcinoma section using GTX02635 Estrogen Receptor alpha antibody [ESR1/2299R].

GTX02635 WB Image

WB analysis of MCF-7 whole cell lysate using GTX02635 Estrogen Receptor alpha antibody [ESR1/2299R].

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 529

Qty