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Estrogen Receptor alpha antibody [ESR1/2299R]

Anti-Estrogen Receptor alpha antibody [ESR1/2299R] used in IHC (Paraffin sections) (IHC-P). GTX02635
Anti-Estrogen Receptor alpha antibody [ESR1/2299R] used in Western Blot (WB). GTX02635

Cat No. GTX02635

Host

Rabbit

Clonality

Monoclonal

Clone Name

ESR1/2299R

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2 μg/ml
IHC-P 1-2 μg/ml

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Observed MW

~67 kDa.

Product Note

This antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human Estrogen Receptor alpha protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1

Cellular Localization

Cytoplasm , Cell membrane , Cell membrane; Single-pass type I membrane protein , Golgi apparatus

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-Estrogen Receptor alpha antibody [ESR1/2299R] used in IHC (Paraffin sections) (IHC-P). GTX02635

GTX02635 IHC-P Image

IHC-P analysis of human breast carcinoma section using GTX02635 Estrogen Receptor alpha antibody [ESR1/2299R].

Anti-Estrogen Receptor alpha antibody [ESR1/2299R] used in Western Blot (WB). GTX02635

GTX02635 WB Image

WB analysis of MCF-7 whole cell lysate using GTX02635 Estrogen Receptor alpha antibody [ESR1/2299R].

REFERENCE

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REVIEW

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