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Estrogen Receptor alpha antibody [HL1081]

Recombinant antibody Comparison Orthogonal Overexpression
Anti-Estrogen Receptor alpha antibody [HL1081] used in Western Blot (WB). GTX636202
Anti-Estrogen Receptor alpha antibody [HL1081] used in IHC (Paraffin sections) (IHC-P). GTX636202
Anti-Estrogen Receptor alpha antibody [HL1081] used in Western Blot (WB). GTX636202
Anti-Estrogen Receptor alpha antibody [HL1081] used in Western Blot (WB). GTX636202
Anti-Estrogen Receptor alpha antibody [HL1081] used in IHC (Paraffin sections) (IHC-P). GTX636202
Anti-Estrogen Receptor alpha antibody [HL1081] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636202

Cat. No. GTX636202

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1081

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Dog
Package
100 μl ($399),
25 μl ($169)
See all Estrogen Receptor alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Product Note

This antibody is specific for human Estrogen Receptor alpha protein, and it does not cross-react with human Estrogen Receptor beta protein.

Predict Reactivity

Mouse, Rat, Bovine, Cat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Estrogen Receptor alpha. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909952

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1

Cellular Localization

Cytoplasm,Cell membrane,Cell membrane; Single-pass type I membrane protein,Golgi apparatus

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-Estrogen Receptor alpha antibody [HL1081] used in Western Blot (WB). GTX636202

GTX636202 WB Image

Comparison Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Estrogen Receptor alpha antibody [HL1081] (GTX636202) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Estrogen Receptor alpha antibody [HL1081] used in IHC (Paraffin sections) (IHC-P). GTX636202

GTX636202 IHC-P Image

Estrogen Receptor alpha antibody [HL1081] detects Estrogen Receptor alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog mammary gland.
Estrogen Receptor alpha stained by Estrogen Receptor alpha antibody [HL1081] (GTX636202) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Estrogen Receptor alpha antibody [HL1081] used in Western Blot (WB). GTX636202

GTX636202 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [HL1081] (GTX636202) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Estrogen Receptor alpha antibody [HL1081] used in Western Blot (WB). GTX636202

GTX636202 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [HL1081] (GTX636202) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Estrogen Receptor alpha antibody [HL1081] used in IHC (Paraffin sections) (IHC-P). GTX636202

GTX636202 IHC-P Image

Estrogen Receptor alpha antibody [HL1081] detects Estrogen Receptor alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Estrogen Receptor alpha stained by Estrogen Receptor alpha antibody [HL1081] (GTX636202) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Estrogen Receptor alpha antibody [HL1081] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636202

GTX636202 ICC/IF Image

Estrogen Receptor alpha antibody [HL1081] detects Estrogen Receptor alpha protein at nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Estrogen Receptor alpha stained by Estrogen Receptor alpha antibody [HL1081] (GTX636202) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations