*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
This antibody is specific for ER alpha protein, and it does not cross react with ER beta protein.
Mouse, Rat, Bovine, Cat, Dog, Pig(>80% identity)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the N-terminus region of human Estrogen Receptor alpha. The exact sequence is proprietary.
Affinity purified by Protein A.
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1
Cytoplasm,Cell membrane,Cell membrane; Single-pass type I membrane protein,Golgi apparatus
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]
GTX636203 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [HL1082] (GTX636203) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636203 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [HL1082] (GTX636203) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX636203 IHC-P Image
Estrogen Receptor alpha antibody [HL1082] detects Estrogen Receptor alpha protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Estrogen Receptor alpha stained by Estrogen Receptor alpha antibody [HL1082] (GTX636203) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min