PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48 and BOND RX - Leica. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Uterine cervix: virtually all squamous and endocervical cells must show a moderate to strong nuclear staining. Tonsil: Scattered follicular dendritic cells in germinal centers and some squamous epithelial cells in tonsil crypts must show an at least weak nuclear ER immunostaining.
Negative Control
Tonsil: B-cells in mantle zones and within germinal centers must be negative.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa495-595) of human ER alpha protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1
Cellular Localization
Nucleus , Cytoplasm , Cell membrane , Cell membrane; Single-pass type I membrane protein , Nucleus , Golgi apparatus
Background
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]
Database
Research Area
DATA IMAGES
![Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474](/templates/default/js/lightbox/images/loading.gif "Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474")
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GTX04474 IHC-P Image
IHC-P analysis of human endometrium (proliferation) tissue using GTX04474 Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™.
Among normal tissues highest levels of estrogen receptor alpha immunostaining occur in epithelial and stromal cells of the endometrium.
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GTX04474 IHC-P Image
IHC-P analysis of human ectocervix tissue using GTX04474 Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™.
An intense estrogen receptor alpha staining is seen in stromal cells and in the majority of squamous epithelial cells of the uterine ectocervix.
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GTX04474 IHC-P Image
IHC-P analysis of human endometrioid carcinoma (EC) tissue using GTX04474 Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™.
Endometroid carcinoma of the ovary with strong estrogen receptor alpha immunostaining of all tumor cells.
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REFERENCE
There are currently no references for Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ (GTX04474). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ (GTX04474). Be the first to share your experience with this product.
![Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474](/upload/website/prouct_img/small/GTX04474/GTX04474_20230728_IHC-P_51_23072722_786.webp "Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474")
![Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474](/upload/website/prouct_img/small/GTX04474/GTX04474_20230728_IHC-P_181_23072723_568.webp "Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474")
![Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474](/upload/website/prouct_img/small/GTX04474/GTX04474_20230728_IHC-P_311_23072723_717.webp "Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474")
![Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474](/upload/website/prouct_img/normal/GTX04474/GTX04474_20230728_IHC-P_51_23072722_786.webp "Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474")
![Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474](/upload/website/prouct_img/normal/GTX04474/GTX04474_20230728_IHC-P_181_23072723_568.webp "Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474")
![Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474](/upload/website/prouct_img/normal/GTX04474/GTX04474_20230728_IHC-P_311_23072723_717.webp "Anti-Estrogen Receptor alpha antibody [MSVA-564R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04474")