APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-5 μg/ml |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
MCF-7
Product Note
This antibody is specific for ER alpha protein, and it does not cross react with ER beta protein.
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human protein expressed in mammalian cells
Purification
Purified by protein A
Purity
>90% determined by SDS-PAGE
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1
Cellular Localization
Cytoplasm , Cell membrane , Cell membrane; Single-pass type I membrane protein , Golgi apparatus
Background
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]
Database
Research Area
DATA IMAGES
![Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805](/templates/default/js/lightbox/images/loading.gif "Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805")
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GTX02805 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [SC1] (GTX02805) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX02805 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [SC1] (GTX02805) diluted at 1:50000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX02805 IHC-P Image
Estrogen Receptor alpha antibody [SC1] detects Estrogen Receptor alpha protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Estrogen Receptor alpha stained by Estrogen Receptor alpha antibody [SC1] (GTX02805) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX02805 ICC/IF Image
ICC/IF analysis of PFA fixed MCF-7 cells by using GTX02805 Estrogen Receptor alpha antibody [SC1].
Dilution : 1-2 μg/ml
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GTX02805 WB Image
WB analysis of MCF-7 cell lysate by using GTX02805 Estrogen Receptor alpha antibody [SC1].
Lane 1-3 were triplicate.
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REFERENCE
There are currently no references for Estrogen Receptor alpha antibody [SC1] (GTX02805). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Estrogen Receptor alpha antibody [SC1] (GTX02805). Be the first to share your experience with this product.
![Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805](/upload/website/prouct_img/small/GTX02805/GTX02805_20201204_WB_w_23053122_604.webp "Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805")
![Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805](/upload/website/prouct_img/small/GTX02805/GTX02805_20201204_WB_B_w_23053122_233.webp "Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805")
![Anti-Estrogen Receptor alpha antibody [SC1] used in IHC (Paraffin sections) (IHC-P). GTX02805](/upload/website/prouct_img/small/GTX02805/GTX02805_20201210_IHC-P_w_23053122_808.webp "Anti-Estrogen Receptor alpha antibody [SC1] used in IHC (Paraffin sections) (IHC-P). GTX02805")
![Anti-Estrogen Receptor alpha antibody [SC1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02805](/upload/website/prouct_img/small/GTX02805/GTX02805_20201130_ICCIF_w_23053122_865.webp "Anti-Estrogen Receptor alpha antibody [SC1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02805")
![Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805](/upload/website/prouct_img/small/GTX02805/GTX02805_20201130_WB_w_23053122_516.webp "Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805")
![Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805](/upload/website/prouct_img/normal/GTX02805/GTX02805_20201204_WB_w_23053122_604.webp "Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805")
![Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805](/upload/website/prouct_img/normal/GTX02805/GTX02805_20201204_WB_B_w_23053122_233.webp "Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805")
![Anti-Estrogen Receptor alpha antibody [SC1] used in IHC (Paraffin sections) (IHC-P). GTX02805](/upload/website/prouct_img/normal/GTX02805/GTX02805_20201210_IHC-P_w_23053122_808.webp "Anti-Estrogen Receptor alpha antibody [SC1] used in IHC (Paraffin sections) (IHC-P). GTX02805")
![Anti-Estrogen Receptor alpha antibody [SC1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02805](/upload/website/prouct_img/normal/GTX02805/GTX02805_20201130_ICCIF_w_23053122_865.webp "Anti-Estrogen Receptor alpha antibody [SC1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02805")
![Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805](/upload/website/prouct_img/normal/GTX02805/GTX02805_20201130_WB_w_23053122_516.webp "Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805")