Estrogen Receptor alpha antibody [SC1]

Cat. No. GTX02805
Cat. No.  GTX02805
Orthogonal Overexpression
Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805
GTX02805 WB Image
Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [SC1] (GTX02805) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805
GTX02805 WB Image

WB analysis of MCF-7 cell lysate by using GTX02805 Estrogen Receptor alpha antibody [SC1].
Lane 1-3 were triplicate.

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Anti-Estrogen Receptor alpha antibody [SC1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02805
GTX02805 ICC/IF Image

ICC/IF analysis of PFA fixed MCF-7 cells by using GTX02805 Estrogen Receptor alpha antibody [SC1].
Dilution : 1-2 μg/ml

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Anti-Estrogen Receptor alpha antibody [SC1] used in IHC (Paraffin sections) (IHC-P). GTX02805
GTX02805 IHC-P Image

Estrogen Receptor alpha antibody [SC1] detects Estrogen Receptor alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Estrogen Receptor alpha stained by Estrogen Receptor alpha antibody [SC1] (GTX02805) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805
GTX02805 WB Image
Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [SC1] (GTX02805) diluted at 1:50000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805
Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805
Anti-Estrogen Receptor alpha antibody [SC1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02805
Anti-Estrogen Receptor alpha antibody [SC1] used in IHC (Paraffin sections) (IHC-P). GTX02805
Anti-Estrogen Receptor alpha antibody [SC1] used in Western Blot (WB). GTX02805
This Estrogen Receptor alpha antibody [SC1] (GTX02805) is a mouse monoclonal antibody validated for detection of Estrogen Receptor alpha by WB, ICC/IF, and IHC-P. Reactivity has been shown in Human and Mouse.
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    SC1
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-P
  • Reactivity
    Human, Mouse
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