APPLICATION
Application Note
ICC/IF: Use at an assay dependent dilution. IP: Use at a concentration of 15 μg/ml. WB: 1:250. Predicted molecular weight: 71.4 kDa. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Product Note
Binding specificity was confirmed in peptide inhibition studies with the intact protein. The antibody does not recognize the native estrogen receptor in the inactive 8S oligomeric state, but reacts efficiently with the activated 4S receptor. In immunoprecipitation studies, this antibody has been shown to only IP the active receptor.
PROPERTIES
Form
Liquid
Buffer
50% Glycerol, PBS pH7.2, 0.1mM PMSF
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide: RAANLWPSPLMIKR conjugated to KLH, corresponding to amino acids 287-300 of Human Estrogen Receptor.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1
Cellular Localization
Cytoplasm , Cell membrane , Cell membrane; Single-pass type I membrane protein , Golgi apparatus
Background
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]
Database
Research Area
DATA IMAGES
![Anti-Estrogen Receptor alpha antibody [h-151] used in Western Blot (WB). GTX13538](/templates/default/js/lightbox/images/loading.gif "Anti-Estrogen Receptor alpha antibody [h-151] used in Western Blot (WB). GTX13538")
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GTX13538 WB Image
Western blot analysis of ESR1:
Lane 1: MWM
Lane 2: Estrogen Receptor 1
GST-tagged ER1 protein control runs at 92 kDa
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REFERENCE
There are currently no references for Estrogen Receptor alpha antibody [h-151] (GTX13538). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Estrogen Receptor alpha antibody [h-151] (GTX13538). Be the first to share your experience with this product.
![Anti-Estrogen Receptor alpha antibody [h-151] used in Western Blot (WB). GTX13538](/upload/website/prouct_img/small/GTX13538/ER-alpha-antibody-h-151_WB_GTX13538-1_w_23060620_402.webp "Anti-Estrogen Receptor alpha antibody [h-151] used in Western Blot (WB). GTX13538")
![Anti-Estrogen Receptor alpha antibody [h-151] used in Western Blot (WB). GTX13538](/upload/website/prouct_img/normal/GTX13538/ER-alpha-antibody-h-151_WB_GTX13538-1_w_23060620_402.webp "Anti-Estrogen Receptor alpha antibody [h-151] used in Western Blot (WB). GTX13538")