GeneTex
United States (US)

Estrogen Receptor alpha antibody

GTX31883_1530_IHC-P_20180221_18121410_859.jpg
GTX31883_3030_WB_20180221_18121410_685.jpg
GTX31883_1529_IHC-P_20180221_18121410_936.jpg

Cat No. GTX31883

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

Rat Brain Tissue Lysate(GTX27942)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Estrogen Receptor alpha antibody was raised against an 18 amino acid peptide near the center of human Estrogen Receptor alpha.The immunogen is located within amino acids 250 - 300 of Estrogen Receptor alpha.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Estrogen Receptor 1,Er,Esr,Esra,Estrr,Era,Nr3A1,Esr1

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES
GTX31883_1530_IHC-P_20180221_18121410_859.jpg

GTX31883 IHC-P Image

IHC-P analysis of rat brain tissue using GTX31883 Estrogen Receptor alpha antibody.
Working concentration : 20 μg/ml

GTX31883_3030_WB_20180221_18121410_685.jpg

GTX31883 WB Image

WB analysis of rat brain tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX31883 Estrogen Receptor alpha antibody.
Working concentration : 1 μg/ml

GTX31883_1529_IHC-P_20180221_18121410_936.jpg

GTX31883 IHC-P Image

IHC-P analysis of rat brain tissue using GTX31883 Estrogen Receptor alpha antibody.
Working concentration : 5 μg/ml

Package List Price ($)
$ 329