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Estrogen Receptor alpha (phospho Ser118) antibody

Anti-Estrogen Receptor alpha (phospho Ser118) antibody used in IHC (Paraffin sections) (IHC-P). GTX32327
Anti-Estrogen Receptor alpha (phospho Ser118) antibody used in Western Blot (WB). GTX32327

Cat No. GTX32327

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

66 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of Estrogen Receptor alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Estrogen Receptor 1,Er,Esr,Esra,Estrr,Era,Nr3A1,Esr1

Cellular Localization

Cell membrane,Cytoplasm,Golgi apparatus,Nucleus

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES
Anti-Estrogen Receptor alpha (phospho Ser118) antibody used in IHC (Paraffin sections) (IHC-P). GTX32327

GTX32327 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32327 Estrogen Receptor alpha (phospho Ser118) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-Estrogen Receptor alpha (phospho Ser118) antibody used in Western Blot (WB). GTX32327

GTX32327 WB Image

WB analysis of EGF-treated MCF7 (A), EGF-treated HeLa (B) whole cell lysates using GTX32327 Estrogen Receptor alpha (phospho Ser118) antibody.

Package List Price ($)
$ 319