Estrogen Receptor alpha (phospho Ser167) antibody

Name: Estrogen Receptor alpha (phospho Ser167) antibody
Brand: GeneTex
SKU: GTX50140
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Estrogen Receptor alpha (phospho Ser167) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50140

Anti-Estrogen Receptor alpha (phospho Ser167) antibody used in Western Blot (WB). GTX50140

Anti-Estrogen Receptor alpha (phospho Ser167) antibody used in IHC (Paraffin sections) (IHC-P). GTX50140

Cat. No. GTX50140

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μl ($399)

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View product citations for antibody GTX50140 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
ICC/IF	1:100-1:200
IHC-P	1:50-1:100
FACS	Assay dependent

Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 167 (L-A-S(p)-T-N) derived from human Estrogen Receptor alpha.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11167711

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1

Cellular Localization

Cytoplasm,Cell membrane,Cell membrane; Single-pass type I membrane protein,Golgi apparatus

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Gene ID: 2099 ESR1
UniProt: P03372 ESR1

Research Area

DATA IMAGES

GTX50140 ICC/IF Image

ICC/IF analysis of methanol-fixed MCF cells using GTX50140 Estrogen Receptor alpha (phospho Ser167) antibody.

GTX50140 WB Image

WB analysis of extracts from MCF-7 cells using GTX50140 Estrogen Receptor alpha (phospho Ser167) antibody.
Lane 1 : Primary antibody pre-incubated with the antigen specific peptide

GTX50140 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50140 Estrogen Receptor alpha (phospho Ser167) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

Application Reference

Ishida N et al. Oncotarget 2018; 9 (25) : 17711-17724 PIK3CA mutation, reduced AKT serine 473 phosphorylation, and increased ERα serine 167 phosphorylation are positive prognostic indicators in postmenopausal estrogen receptor-positive early breast cancer.

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REVIEW