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Estrogen Receptor beta 2 antibody [57/3]

Anti-Estrogen Receptor beta 2 antibody [57/3] used in IHC (Paraffin sections) (IHC-P). GTX41213
Anti-Estrogen Receptor beta 2 antibody [57/3] used in IHC (Paraffin sections) (IHC-P). GTX41213

Cat No. GTX41213

Host Mouse
Clonality Monoclonal
Clone Name 57/3
Isotype IgG1
Application IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1/50-1/500

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. Glycine/ EDTA preparations have also been used with success in some tissues.

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes the estrogen receptor beta 2 isoform. No cross-reactivity is seen with estrogen receptor beta 1 isoform.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide derived from the C terminus of the human estrogen receptor beta 2 isoform, CGMKMETLLPEATMEQ.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Estrogen Receptor 2,Er-Beta,Esr-Beta,Esrb,Estrb,Erb,Nr3A2,Esr2

Background

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Estrogen Receptor beta 2 antibody [57/3] used in IHC (Paraffin sections) (IHC-P). GTX41213

GTX41213 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX41213 Estrogen Receptor beta 2 antibody. Antigen retrieval using citrate buffer at pH6.2.

Anti-Estrogen Receptor beta 2 antibody [57/3] used in IHC (Paraffin sections) (IHC-P). GTX41213

GTX41213 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX41213 Estrogen Receptor beta 2 antibody. Antigen retrieval using citrate buffer at pH6.2.

Package List Price ($)
$ 469