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Estrogen Receptor beta 5 antibody [PPG5/25]

Anti-Estrogen Receptor beta 5 antibody [PPG5/25] used in IHC (Paraffin sections) (IHC-P). GTX04767

Cat. No. GTX04767

Host

Mouse

Clonality

Monoclonal

Clone Name

PPG5/25

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($529)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2-4 μg/mL
ICC/IF 1-2 μg/mL
IHC-P 1-2 μg/mL

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

colon or colon carcinoma, HCT cells, Normal liver

PROPERTIES

Form

Liquid

Buffer

10 mM PBS, 0.05% BSA (Please contact us for PBS only format).

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Tuberculin conjugated synthetic peptide (LLSHVRHARYAP); derived from the C-terminus of human Estrogen Receptor beta 5

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERBETA,ESR2,ESRB,ESRBETA,ESTRB,Erb,NR3A2,estrogen receptor 2,ER beta,Estrogen Receptor beta

Cellular Localization

Nucleus

Background

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Estrogen Receptor beta 5 antibody [PPG5/25] used in IHC (Paraffin sections) (IHC-P). GTX04767

GTX04767 IHC-P Image

IHC-P analysis of human breast tissue using GTX04767 Estrogen Receptor beta 5 antibody [PPG5/25].

REFERENCE

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REVIEW

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Package List Price ($)
$ 529