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Estrogen Receptor beta antibody [14C8]

Anti-Estrogen Receptor beta antibody [14C8] used in IHC (Paraffin sections) (IHC-P). GTX70174
Anti-Estrogen Receptor beta antibody [14C8] used in Immunohistochemistry (IHC). GTX70174
Anti-Estrogen Receptor beta antibody [14C8] used in Western Blot (WB). GTX70174

Cat No. GTX70174

Host Mouse
Clonality Monoclonal
Clone Name 14C8
Isotype IgG2b
Application WB, ICC/IF, IHC-P, FACS, Dot, ChIP assay, IHC
Reactivity Human, Mouse, Monkey
APPLICATION

Application Note

For IHC, a recommended protocol is provided. For WB: Use at a concentration of 1-5 μg/ml. For ChIP: Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

58-60 kDa. ( Note )

Positive Control

Myc-DDK-tagged ESR2-transfected 293T

Specificity/Sensitivity

Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody.
Knockdown/Knockout validation was supported by references (PMID:27323858)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 1-153 of human ER-beta expressed in E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Estrogen Receptor 2,Er-Beta,Esr-Beta,Esrb,Estrb,Erb,Nr3A2,Esr2

Background

The human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domain has diverged significantly. Similar in function to ER-alpha ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's).

Database

Research Area

DATA IMAGES
Anti-Estrogen Receptor beta antibody [14C8] used in IHC (Paraffin sections) (IHC-P). GTX70174

GTX70174 IHC-P Image

Estrogen Receptor beta antibody [14C8] detects Estrogen Receptor beta protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Estrogen Receptor beta stained by Estrogen Receptor beta antibody [14C8] (GTX70174) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Estrogen Receptor beta antibody [14C8] used in Immunohistochemistry (IHC). GTX70174

GTX70174 IHC Image

B. Infiltrating lobular carcinoma of the breast.

Anti-Estrogen Receptor beta antibody [14C8] used in Western Blot (WB). GTX70174

GTX70174 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [14C8] (GTX70174) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Estrogen Receptor beta antibody [14C8] Cat No. GTX70174

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 13-Feb-2019
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Cells differentiated from neural stem cells
Blocking : 10% goat serum in PBS, 25°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Tritonx-100 in PBS
Primary Antibody : 1:500, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319