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Estrogen Receptor beta antibody [4D2]

Anti-Estrogen Receptor beta antibody [4D2] used in IHC (Paraffin sections) (IHC-P). GTX70176
Anti-Estrogen Receptor beta antibody [4D2] used in Western Blot (WB). GTX70176
Anti-Estrogen Receptor beta antibody [4D2] used in Western Blot (WB). GTX70176

Cat No. GTX70176

Host Mouse
Clonality Monoclonal
Clone Name 4D2
Isotype IgG2b
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 5 μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

58-60 kDa. ( Note )

Positive Control

Myc-DDK-tagged ESR2-transfected 293T , Recombinant fusion protein
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 1-153 of human ER-§ expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Estrogen Receptor 2,Er-Beta,Esr-Beta,Esrb,Estrb,Erb,Nr3A2,Esr2

Background

The human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domain has diverged significantly. Similar in function to ER-alpha ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's).

Database

Research Area

DATA IMAGES
Anti-Estrogen Receptor beta antibody [4D2] used in IHC (Paraffin sections) (IHC-P). GTX70176

GTX70176 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using ER beta(GTX70176) antibody at 1:200 dilution.

Anti-Estrogen Receptor beta antibody [4D2] used in Western Blot (WB). GTX70176

GTX70176 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [4D2] (GTX70176) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Estrogen Receptor beta antibody [4D2] used in Western Blot (WB). GTX70176

GTX70176 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: PC-3
C: U87-MG
D: SK-N-SH
7.5% SDS PAGE
GTX70176 diluted at 1:500

REFERENCE
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REVIEW
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Package List Price ($)
$ 319