APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 - 5 μg/ml |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Myc-DDK-tagged ESR2-transfected 293T , Recombinant fusion protein
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Amino acids 1-153 of human ER-§ expressed in E. coli.
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Unconjugated
RRID
AB_370369
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
estrogen receptor 2 , ER-BETA , ESR-BETA , ESRB , ESTRB , Erb , NR3A2 , ODG8
Cellular Localization
Nucleus
Background
This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX70176 IHC-P Image
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using ER beta(GTX70176) antibody at 1:200 dilution.
|
|
GTX70176 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [4D2] (GTX70176) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for Estrogen Receptor beta antibody [4D2] (GTX70176). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Estrogen Receptor beta antibody [4D2] (GTX70176). Be the first to share your experience with this product.