GeneTex
United States (US)

Estrogen Receptor beta antibody [6A12]

GTX70178_1_20180126_WB_B_18121411_449.jpg

Cat No. GTX70178

Host Mouse
Clonality Monoclonal
Clone Name 6A12
Isotype IgG2a
Application WB, ICC/IF, ChIP assay, IHC
Reactivity Human
APPLICATION

Application Note

For WB: Use at a concentration of 1 - 5 μg/ml. Predicted molecular weight: 59 kDa.For ICC/IF:Use at an dependent assay. For ChIP:Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

58-60 kDa. ( Note )

Positive Control

Myc-DDK-tagged ESR2-transfected 293T , Recombinant fusion protein

Specificity/Sensitivity

6A12 is specific for human ER-beta.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 1-153 of human ER-§ expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ESR-BETA, ESR2, 2100, ESRB, Q92731, Erb, ERBETA, 5p152, 601663, ER-BETA, NR3A2, ER beta, ESRBETA, ESR BETA

Background

The human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domain has diverged significantly. Similar in function to ER-alpha ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's).

Database

Research Area

DATA IMAGES
GTX70178_1_20180126_WB_B_18121411_449.jpg

GTX70178 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [6A12] (GTX70178) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
Application Reference
Package List Price ($)
$ 319