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Estrogen Receptor beta antibody [6A12]

Anti-Estrogen Receptor beta antibody [6A12] used in Western Blot (WB). GTX70178

Cat No. GTX70178

Host

Mouse

Clonality

Monoclonal

Clone Name

6A12

Isotype

IgG2a

Application

WB, ICC/IF, ChIP assay, IHC

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 5 μg/ml
ICC/IF Assay dependent
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Myc-DDK-tagged ESR2-transfected 293T , Recombinant fusion protein

Product Note

6A12 is specific for human ER-beta.

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 1-153 of human ER-§ expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_370371

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

estrogen receptor 2 , ER-BETA , ESR-BETA , ESRB , ESTRB , Erb , NR3A2 , ODG8

Cellular Localization

Nucleus

Background

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Estrogen Receptor beta antibody [6A12] used in Western Blot (WB). GTX70178

GTX70178 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [6A12] (GTX70178) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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Application Reference