GeneTex

  • Country / Location Selection

United States (US)

Estrogen Receptor beta antibody [6B12]

Anti-Estrogen Receptor beta antibody [6B12] used in Western Blot (WB). GTX70180

Cat. No. GTX70180

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6B12

Isotype

 IgG1

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all Estrogen Receptor beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Myc-DDK-tagged ESR2-transfected 293T

Product Note

6B12 is specific for human ER-beta.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 1-153 of human ER-§ expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_370373

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

estrogen receptor 2 , ER-BETA , ESR-BETA , ESRB , ESTRB , Erb , NR3A2 , ODG8

Cellular Localization

Nucleus

Background

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Estrogen Receptor beta antibody [6B12] used in Western Blot (WB). GTX70180

GTX70180 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [6B12] (GTX70180) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Dietrich W et al. Fertil Steril 2011; 95 (4) : 1247-55.e1-2 Testosterone dependent androgen receptor stabilization and activation of cell proliferation in primary human myometrial microvascular endothelial cells.
Demyanets S et al. Thromb Haemost 2006; 95 (1) : 107-16 The estrogen * 17beta-dihydroequilenin counteracts interleukin-1alpha induced expression of inflammatory mediators in human endothelial cells in vitro via NF-kappaB pathway.
Application : WB
Reactivity : Human
Tschugguel W et al. J Clin Endocrinol Metab 2003; 88 (5) : 139341 Differential regulation of proteasome-dependent estrogen receptor alpha and beta turnover in cultured human uterine artery endothelial cells.
Application : WB
Reactivity : Human
Fuchsjager-Mayrl G et al. Invest Ophthalmol Vis Sci 2002; 43 (9) : 343786 Identification of estrogen and progesterone receptor mRNA expression in the conjunctiva of premenopausal women.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Estrogen Receptor beta antibody [6B12] (GTX70180). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
Custom Conjugations