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Estrogen Receptor beta antibody [ERb455]

Anti-Estrogen Receptor beta antibody [ERb455] used in Western Blot (WB). GTX34711
Anti-Estrogen Receptor beta antibody [ERb455] used in Flow cytometry (FACS). GTX34711
Anti-Estrogen Receptor beta antibody [ERb455] used in IHC (Paraffin sections) (IHC-P). GTX34711
Anti-Estrogen Receptor beta antibody [ERb455] used in IHC (Paraffin sections) (IHC-P). GTX34711
Anti-Estrogen Receptor beta antibody [ERb455] used in IHC (Paraffin sections) (IHC-P). GTX34711
Anti-Estrogen Receptor beta antibody [ERb455] used in IHC (Paraffin sections) (IHC-P). GTX34711
Anti-Estrogen Receptor beta antibody [ERb455] used in Flow cytometry (FACS). GTX34711

Cat No. GTX34711

Host

Mouse

Clonality

Monoclonal

Clone Name

ERb455

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Rat, Sheep, Pig, Monkey, Horse
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

C-terminus fragment of recombinant human estrogen receptor beta protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ERBETA , ESR2 , ESRB , ESRBETA , ESTRB , Erb , NR3A2 , estrogen receptor 2 , ER beta , Estrogen Receptor beta

Cellular Localization

Nucleus

Background

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Estrogen Receptor beta antibody [ERb455] used in Western Blot (WB). GTX34711

GTX34711 WB Image

WB analysis of MCF-7 cell lysate using GTX34711 Estrogen Receptor beta antibody [ERb455].

Anti-Estrogen Receptor beta antibody [ERb455] used in Flow cytometry (FACS). GTX34711

GTX34711 FACS Image

FACS analysis of MCF-7 cells using GTX34711 Estrogen Receptor beta antibody [ERb455].
Black : Blank
Green : Isotype Control
Red : Primary antibody

Anti-Estrogen Receptor beta antibody [ERb455] used in IHC (Paraffin sections) (IHC-P). GTX34711

GTX34711 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX34711 Estrogen Receptor beta antibody [ERb455].

Anti-Estrogen Receptor beta antibody [ERb455] used in IHC (Paraffin sections) (IHC-P). GTX34711

GTX34711 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX34711 Estrogen Receptor beta antibody [ERb455].

Anti-Estrogen Receptor beta antibody [ERb455] used in IHC (Paraffin sections) (IHC-P). GTX34711

GTX34711 IHC-P Image

IHC-P analysis of human gastric carcinoma tissue using GTX34711 Estrogen Receptor beta antibody [ERb455].

Anti-Estrogen Receptor beta antibody [ERb455] used in IHC (Paraffin sections) (IHC-P). GTX34711

GTX34711 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX34711 Estrogen Receptor beta antibody [ERb455].

Anti-Estrogen Receptor beta antibody [ERb455] used in Flow cytometry (FACS). GTX34711

GTX34711 FACS Image

FACS analysis of BT474 cells using GTX34711 Estrogen Receptor beta antibody [ERb455].
Black : Blank
Grey : Isotype Control
Green : Primary antibody

REFERENCE

There are currently no references for Estrogen Receptor beta antibody [ERb455] (GTX34711). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Estrogen Receptor beta antibody [ERb455] (GTX34711). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339