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Estrogen Receptor beta antibody [ERb455]

Anti-Estrogen Receptor beta antibody [ERb455] used in Flow cytometry (FACS). GTX34711
Anti-Estrogen Receptor beta antibody [ERb455] used in IHC (Paraffin sections) (IHC-P). GTX34711
Anti-Estrogen Receptor beta antibody [ERb455] used in IHC (Paraffin sections) (IHC-P). GTX34711
Anti-Estrogen Receptor beta antibody [ERb455] used in IHC (Paraffin sections) (IHC-P). GTX34711

Cat No. GTX34711

Host Mouse
Clonality Monoclonal
Clone Name ERb455
Isotype IgG2a
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Mouse, Rat, Sheep, Pig, Monkey, Horse
Package
100 μg ($429)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT
FACS Assay dependent

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Specificity/Sensitivity

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.
PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

C-terminus fragment of recombinant human estrogen receptor beta protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Estrogen Receptor 2 , Er-Beta , Esr-Beta , Esrb , Estrb , Erb , Nr3A2 , Esr2

Background

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Estrogen Receptor beta antibody [ERb455] used in Flow cytometry (FACS). GTX34711

GTX34711 FACS Image

Flow Cytometry for human ER-beta on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled ER-beta1 Monoclonal Antibody (ERb455).

Anti-Estrogen Receptor beta antibody [ERb455] used in IHC (Paraffin sections) (IHC-P). GTX34711

GTX34711 IHC-P Image

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with ER-beta1 Monoclonal Antibody (ERb455).

Anti-Estrogen Receptor beta antibody [ERb455] used in IHC (Paraffin sections) (IHC-P). GTX34711

GTX34711 IHC-P Image

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with ER-beta1 Monoclonal Antibody (ERb455).

Anti-Estrogen Receptor beta antibody [ERb455] used in IHC (Paraffin sections) (IHC-P). GTX34711

GTX34711 IHC-P Image

Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with ER-beta1 Monoclonal Antibody (ERb455).

REFERENCE
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REVIEW
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Package List Price ($)
$ 429