APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
C-terminus fragment of recombinant human estrogen receptor beta protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ERBETA , ESR2 , ESRB , ESRBETA , ESTRB , Erb , NR3A2 , estrogen receptor 2 , ER beta , Estrogen Receptor beta
Cellular Localization
Nucleus
Background
This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712](/templates/default/js/lightbox/images/loading.gif "Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712")
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GTX34712 IHC-P Image
IHC-P analysis of human bladder carcinoma tissue using GTX34712 Estrogen Receptor beta antibody [ESR2/686].
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GTX34712 IHC-P Image
IHC-P analysis of human gastric carcinoma tissue using GTX34712 Estrogen Receptor beta antibody [ESR2/686].
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GTX34712 FACS Image
FACS analysis of BT474 cells using GTX34712 Estrogen Receptor beta antibody [ESR2/686].
Black : Blank
Grey : Isotype Control
Green : Primary antibody
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GTX34712 IHC-P Image
IHC-P analysis of human ovarian carcinoma tissue using GTX34712 Estrogen Receptor beta antibody [ESR2/686].
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GTX34712 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX34712 Estrogen Receptor beta antibody [ESR2/686].
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GTX34712 FACS Image
FACS analysis of MCF-7 cells using GTX34712 Estrogen Receptor beta antibody [ESR2/686].
Black : Blank
Green : Isotype Control
Red : Primary antibody
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REFERENCE
There are currently no references for Estrogen Receptor beta antibody [ESR2/686] (GTX34712). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Estrogen Receptor beta antibody [ESR2/686] (GTX34712). Be the first to share your experience with this product.
![Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712](/upload/website/prouct_img/small/GTX34712/GTX34712_20200115_IHC-P_301_w_23060801_197.webp "Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712")
![Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712](/upload/website/prouct_img/small/GTX34712/GTX34712_20200115_IHC-P_582_w_23060801_686.webp "Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712")
![Anti-Estrogen Receptor beta antibody [ESR2/686] used in Flow cytometry (FACS). GTX34712](/upload/website/prouct_img/small/GTX34712/GTX34712_20200115_FACS_62_w_23060801_361.webp "Anti-Estrogen Receptor beta antibody [ESR2/686] used in Flow cytometry (FACS). GTX34712")
![Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712](/upload/website/prouct_img/small/GTX34712/GTX34712_20200115_IHC-P_821_w_23060801_917.webp "Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712")
![Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712](/upload/website/prouct_img/small/GTX34712/GTX34712_20200115_IHC-P_353_w_23060801_349.webp "Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712")
![Anti-Estrogen Receptor beta antibody [ESR2/686] used in Flow cytometry (FACS). GTX34712](/upload/website/prouct_img/small/GTX34712/GTX34712_20200115_FACS_1550_w_23060801_561.webp "Anti-Estrogen Receptor beta antibody [ESR2/686] used in Flow cytometry (FACS). GTX34712")
![Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712](/upload/website/prouct_img/normal/GTX34712/GTX34712_20200115_IHC-P_301_w_23060801_197.webp "Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712")
![Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712](/upload/website/prouct_img/normal/GTX34712/GTX34712_20200115_IHC-P_582_w_23060801_686.webp "Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712")
![Anti-Estrogen Receptor beta antibody [ESR2/686] used in Flow cytometry (FACS). GTX34712](/upload/website/prouct_img/normal/GTX34712/GTX34712_20200115_FACS_62_w_23060801_361.webp "Anti-Estrogen Receptor beta antibody [ESR2/686] used in Flow cytometry (FACS). GTX34712")
![Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712](/upload/website/prouct_img/normal/GTX34712/GTX34712_20200115_IHC-P_821_w_23060801_917.webp "Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712")
![Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712](/upload/website/prouct_img/normal/GTX34712/GTX34712_20200115_IHC-P_353_w_23060801_349.webp "Anti-Estrogen Receptor beta antibody [ESR2/686] used in IHC (Paraffin sections) (IHC-P). GTX34712")
![Anti-Estrogen Receptor beta antibody [ESR2/686] used in Flow cytometry (FACS). GTX34712](/upload/website/prouct_img/normal/GTX34712/GTX34712_20200115_FACS_1550_w_23060801_561.webp "Anti-Estrogen Receptor beta antibody [ESR2/686] used in Flow cytometry (FACS). GTX34712")