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Estrogen Receptor beta antibody [N2C2], Internal

KO/KD Overexpression
GTX110607_42599_20160908_WB_19011507_834.jpg
GTX110607_WB_M_19011507_616.jpg
GTX110607_40044_CT_WB_19011507_605.jpg

Cat No. GTX110607

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , GC1 , GC1 co-transfected ER-beta (NM_207707) , *U2OS , *HEC-1A

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:26079540, PMID:22052999, PMID:24148819, PMID:21492444)

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.34mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Estrogen Receptor beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Estrogen Receptor 2,Er-Beta,Esr-Beta,Esrb,Estrb,Erb,Nr3A2,Esr2

Cellular Localization

Nucleus

Background

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX110607_42599_20160908_WB_19011507_834.jpg

GTX110607 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [N2C2], Internal (GTX110607) diluted at 1:1000.

GTX110607_WB_M_19011507_616.jpg

GTX110607 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX110607 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110607_40044_CT_WB_19011507_605.jpg

GTX110607 WB Image

Overexpression

Sample (60 µg of whole cell lysate)
A: GC1
B: GC1 co-transfected ER-beta (NM_207707)
12% SDS PAGE
GTX110607 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Estrogen Receptor beta antibody [N2C2], Internal Cat No. GTX110607

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 05-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEC-1A cells
Amount used : 30μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 17Hr
Date : Anonymous submitted on 09-Dec-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30μg
Blocking : 5% milk in TBST, 37°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169
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