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Estrogen Receptor beta antibody

Anti-Estrogen Receptor beta antibody used in Western Blot (WB). GTX25786
Anti-Estrogen Receptor beta antibody used in IHC (Paraffin sections) (IHC-P). GTX25786

Cat. No. GTX25786

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, Gel supershift assays

Reactivity

 Human, Rat, Pig, Primate
Package
50 μg ($399)
See all Estrogen Receptor beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
IHC-P 2-5 μg/mL
IHC-Fr 2-5 μg/mL
Gel supershift assays Assay dependent
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues C(459) S P A E D S K S K E G S Q N P Q S Q(477) of human ER beta.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

estrogen receptor 2 , ER-BETA , ESR-BETA , ESRB , ESTRB , Erb , NR3A2 , ODG8

Cellular Localization

Nucleus

Background

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Estrogen Receptor beta antibody used in Western Blot (WB). GTX25786

GTX25786 WB Image

WB analysis of full-length human ER alpha recombinant protein and ER beta protein using GTX25786 Estrogen Receptor beta antibody at 1:1000 (left panel) or Estrogen Receptor alpha antibody (right panel).

Anti-Estrogen Receptor beta antibody used in IHC (Paraffin sections) (IHC-P). GTX25786

GTX25786 IHC-P Image

IHC-P analysis of rat prostate tissue using GTX25786 Estrogen Receptor beta antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399