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Exportin 5 antibody

Anti-Exportin 5 antibody used in Western Blot (WB). GTX130727
Anti-Exportin 5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130727

Cat. No. GTX130727

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Frog
Package
100 μl ($399),
25 μl ($169)
See all Exportin 5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , K562

Predict Reactivity

Mouse, Rat, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Exportin 5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886329

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

exportin 5 , exp5

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. The encoded protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-Exportin 5 antibody used in Western Blot (WB). GTX130727

GTX130727 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Exportin 5 antibody (GTX130727) diluted at 1:1000.

Anti-Exportin 5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130727

GTX130727 ICC/IF Image

XPO5 antibody detects XPO5 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XPO5 protein stained by XPO5 antibody (GTX130727) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Hadj-Moussa H et al. Cell Mol Life Sci 2018; (Epub) Micromanaging freeze tolerance: the biogenesis and regulation of neuroprotective microRNAs in frozen brains.
Application : WB
Reactivity : Frog
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169