APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human EZR protein, and it does not cross react with human RDX, MSN, and NF2 protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human Ezrin.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ezrin , CVIL , CVL , HEL-S-105 , VIL2
Cellular Localization
Apical cell membrane,Cytoplasm, cytoskeleton
Background
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490](/templates/default/js/lightbox/images/loading.gif "Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490")
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GTX638490 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [HL2327] (GTX638490) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638490 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [HL2327] (GTX638490) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638490 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [HL2327] (GTX638490) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX638490 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [HL2327] (GTX638490) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for Ezrin antibody [HL2327] (GTX638490). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Ezrin antibody [HL2327] (GTX638490). Be the first to share your experience with this product.
![Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490](/upload/website/prouct_img/small/GTX638490/GTX638490_T-45005_20230929_WB_M_R_23100319_774.webp "Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490")
![Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490](/upload/website/prouct_img/small/GTX638490/GTX638490_T-45005_20231013_WB_multiple_B_23101921_482.webp "Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490")
![Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490](/upload/website/prouct_img/small/GTX638490/GTX638490_45243_20231201_WB_23120519_566.webp "Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490")
![Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490](/upload/website/prouct_img/small/GTX638490/GTX638490_45243_20240119_WB_D_24012217_535.webp "Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490")
![Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490](/upload/website/prouct_img/normal/GTX638490/GTX638490_T-45005_20230929_WB_M_R_23100319_774.webp "Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490")
![Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490](/upload/website/prouct_img/normal/GTX638490/GTX638490_T-45005_20231013_WB_multiple_B_23101921_482.webp "Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490")
![Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490](/upload/website/prouct_img/normal/GTX638490/GTX638490_45243_20231201_WB_23120519_566.webp "Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490")
![Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490](/upload/website/prouct_img/normal/GTX638490/GTX638490_45243_20240119_WB_D_24012217_535.webp "Anti-Ezrin antibody [HL2327] used in Western Blot (WB). GTX638490")