Specificity test was performed by coating the plate with EZR peptide, EZR (phospho Thr567) peptide, MSN peptide, MSN (phospho Thr558) peptide, RDX peptide, and RDX (phospho Thr564) peptide (62.5 ng/mL). Coated peptides were probed with Ezrin (phospho Thr567) + Radixin (Thr564) + Moesin (Thr558) Antibody [HL2909] (GTX640252) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin (phospho Thr567) + Radixin (Thr564) + Moesin (Thr558) Antibody [HL2909] (GTX640252) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.