Ezrin (phospho Thr567) antibody

Cat. No. GTX79080

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human
Package	100 μg ($399)

See all Ezrin products

Datasheet

View product citations for antibody GTX79080 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000

Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of threonine 566 (Y-K-Tp-L-R).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11168826

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CVIL,CVL,EZR,HELS105,VIL2,ezrin,Ezrin

Cellular Localization

Apical cell membrane,Cell projection,Microvillus membrane,Ruffle membrane,Cytoplasm,Cell cortex,Cytoskeleton

Background

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 7430 EZR
UniProt: P15311 EZR

Research Area

DATA IMAGES

GTX79080 WB Image

WB analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30min) using GTX79080 Ezrin (phospho Thr567) antibody (Lane 3 and 4) and Ezrin antibody (Lane 1 and 2).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 399

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