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Ezrin (phospho Tyr354) antibody

Anti-Ezrin (phospho Tyr354) antibody used in Western Blot (WB). GTX50135
Anti-Ezrin (phospho Tyr354) antibody used in IHC (Paraffin sections) (IHC-P). GTX50135

Cat. No. GTX50135

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all Ezrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from human Ezrin.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ezrin , CVIL , CVL , HEL-S-105 , VIL2

Cellular Localization

Apical cell membrane,Cytoplasm, cytoskeleton

Background

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ezrin (phospho Tyr354) antibody used in Western Blot (WB). GTX50135

GTX50135 WB Image

WB analysis of extracts from 293 cells untreated or treated with EGF using GTX50135 Ezrin (phospho Tyr354) antibody.

Anti-Ezrin (phospho Tyr354) antibody used in IHC (Paraffin sections) (IHC-P). GTX50135

GTX50135 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50135 Ezrin (phospho Tyr354) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399