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Ezrin (phospho Tyr353) antibody

Anti-Ezrin (phospho Tyr353) antibody used in IHC (Paraffin sections) (IHC-P). GTX50135
Anti-Ezrin (phospho Tyr353) antibody used in Western Blot (WB). GTX50135

Cat No. GTX50135

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from human Ezrin.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ezrin , CVIL , CVL , HEL-S-105 , VIL2

Cellular Localization

Apical cell membrane,Cytoplasm, cytoskeleton

Background

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ezrin (phospho Tyr353) antibody used in IHC (Paraffin sections) (IHC-P). GTX50135

GTX50135 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50135 Ezrin (phospho Tyr353) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-Ezrin (phospho Tyr353) antibody used in Western Blot (WB). GTX50135

GTX50135 WB Image

WB analysis of extracts from 293 cells untreated or treated with EGF using GTX50135 Ezrin (phospho Tyr353) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf