GeneTex
United States (US)

F-Actin antibody [NH3]

GTX20205__20161004_IFA_18121410_445.jpg

Cat No. GTX20205

Host Mouse
Clonality Monoclonal
Clone Name NH3
Isotype IgM
Application WB, ICC/IF, IHC-Fr, FACS, ELISA
Reactivity Human, Mouse, Rat, Rabbit
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/100-1/500
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
FACS 1/10
ELISA Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Specificity/Sensitivity

This antibody recognizes human Filamentous actin (F-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human monocytes and U937 cell line.

Purification

Purified IgM
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PS1TP5BP1 Antibody , BRWS1 Antibody , ACTB Antibody , F-actin Antibody , F actin Antibody , Filamentous actin Antibody

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]
DATA IMAGES
GTX20205__20161004_IFA_18121410_445.jpg

GTX20205 ICC/IF Image

F-Actin antibody [NH3] detects F-Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: F-Actin protein stained by F-Actin antibody [NH3] (GTX20205) diluted at 1:250.
Green: NF-M, stained by NF-M antibody (GTX85461) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
Package List Price ($)
$ 459