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F-Actin antibody [NH3]

Anti-F-Actin antibody [NH3] used in Western Blot (WB). GTX76100

Cat. No. GTX76100

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 NH3

Isotype

 IgM

Application

 WB, IHC-Fr, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Rabbit
Package
25 μg ($189)
See all F-actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Fr Assay dependent
FACS 1/10
ELISA Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Product Note

This antibody recognizes human Filamentous actin (F-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human monocytes and U937 cell line.

Purification

Purified IgM
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_377199

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PS1TP5BP1 Antibody , BRWS1 Antibody , ACTB Antibody , F-actin Antibody , F actin Antibody , Filamentous actin Antibody

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

Research Area

DATA IMAGES

Anti-F-Actin antibody [NH3] used in Western Blot (WB). GTX76100

GTX76100 WB Image

WB analysis of HeLa cell lysates using GTX76100 F-Actin antibody [NH3].

REFERENCE

Clone Reference

Xuan Y et al. Int J Nanomedicine 2023; 18:917-932 The 3D-Printed Ordered Bredigite Scaffold Promotes Pro-Healing of Critical-Sized Bone Defects by Regulating Macrophage Polarization.
Zou Liting et al. Chinese Journal of Cell Biology 2015; Functional Characterization of CX3CL1 in Altering the Cytoskeleton of Human Umbilical Vein Endothelial Cells.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 189