GeneTex
United States (US)

F2RL3 / PAR-4 (cleaved Gly48) antibody

PAR4-cleaved-Gly48-antibody-GTX86937-WB-1_18121411_249.jpg

Cat No. GTX86937

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:1000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

33 kDa. ( Note )

Specificity/Sensitivity

PAR4 (Cleaved-Gly48) Antibody detects endogenous levels of fragment of activated PAR4 resulting from cleavage adjacent to Gly48.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human PAR4.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

F2R Like Thrombin Or Trypsin Receptor 3,Par4,F2Rl3

Background

Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.

Database

DATA IMAGES
PAR4-cleaved-Gly48-antibody-GTX86937-WB-1_18121411_249.jpg

GTX86937 WB Image

Western blot analysis of extracts from NIH-3T3 cells, treated with etoposide 25uM 1h, using PAR4 (Cleaved-Gly48) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339