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F4/80 antibody [BM8.1] (PE-Cy7)

Anti-F4/80 antibody [BM8.1] (PE-Cy7) used in Flow cytometry (FACS). GTX01470-10

Cat No. GTX01470-10

Host

Rat

Clonality

Monoclonal

Clone Name

BM8.1

Isotype

IgG2b

Application

FACS

Reactivity

Mouse
Package
100 μg ($339)
PRODUCT

Summary

The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10 mM NaH₂PO₄ (pH 7.2), 150 mM NaCl, 0.09% sodium azide, 0.1% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin-Cyanine7 (PE-Cy7)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-F4/80 antibody [BM8.1] (PE-Cy7) used in Flow cytometry (FACS). GTX01470-10

GTX01470-10 FACS Image

FACS analysis of mouse C57Bl/6 bone marrow cells using GTX01470-10 F4/80 antibody [BM8.1] (PE-Cy7).
Right panel : co-stained with F4/80 antibody [BM8.1] (PE-Cy7) and Mouse CD11b antibody (APC)
Left panel : co-stained with isotype control and Mouse CD11b antibody (APC)
antibody amount : 0.25 μg (5 μl)

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf