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F4/80 antibody [BM8.1]

Cat. No. GTX01470

Reference 5

Host

 Rat

Clonality

 Monoclonal

Clone Name

 BM8.1

Isotype

 IgG2b

Application

 IHC-P, IHC-Fr, FACS

Reactivity

 Mouse
Package
100 μg ($159)
See all F4/80 products
PRODUCT

Summary

The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Purity

> 90% (determined by SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cell membrane

Database

Research Area

REFERENCE

Clone Reference

2013; Inflammatory monocytes are critical for induction of a polysaccharide-specific antibody response to an intact bacterium.
Application : FACS
Reactivity : Mouse
2013; Macrophage-specific TLR2 signaling mediates pathogen-induced TNF-dependent inflammatory oral bone loss.
Application : FACS
Reactivity : Mouse
2013; In vivo ablation of plasmacytoid dendritic cells inhibits autoimmunity through expansion of myeloid-derived suppressor cells.
Application : FACS
Reactivity : Mouse
2010; IL-33 induces IL-13-dependent cutaneous fibrosis.
Application : IHC-P
Reactivity : Mouse
2005; Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.
Application : IHC-Fr
Reactivity : Mouse
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 159