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F4/80 antibody [BM8.1]

Cat No. GTX01470

Host

Rat

Clonality

Monoclonal

Clone Name

BM8.1

Isotype

IgG2b

Application

IHC-P, IHC-Fr, FACS

Reactivity

Mouse
Package
100 μg ($149)
PRODUCT

Summary

The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10 mM NaH₂PO₄ (pH 7.2), 150 mM NaCl, 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Purity

> 90% (determined by SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Cell membrane

Database

Research Area

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf