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F4/80 antibody [C2C3], C-term

Anti-F4/80 antibody [C2C3], C-term used in Western Blot (WB). GTX101895
Anti-F4/80 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101895
Anti-F4/80 antibody [C2C3], C-term used in Western Blot (WB). GTX101895
Anti-F4/80 antibody [C2C3], C-term used in Western Blot (WB). GTX101895

Cat No. GTX101895

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , rat liver

Predict Reactivity

Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human EMR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

adhesion G protein-coupled receptor E1 , EMR1 , TM7LN3

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-F4/80 antibody [C2C3], C-term used in Western Blot (WB). GTX101895

GTX101895 WB Image

EMR1 antibody [C2C3], C-term detects EMR1 protein by western blot analysis.
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
C. 30 µg K562 whole cell lysate/extract
7.5% SDS-PAGE
EMR1 antibody [C2C3], C-term (GTX101895) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-F4/80 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101895

GTX101895 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using EMR1(GTX101895) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-F4/80 antibody [C2C3], C-term used in Western Blot (WB). GTX101895

GTX101895 WB Image

EMR1 antibody [C2C3], C-term detects EMR1 protein by western blot analysis.
A. 50 µg rat liver lysate/extract
7.5% SDS-PAGE
EMR1 antibody [C2C3], C-term (GTX101895) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-F4/80 antibody [C2C3], C-term used in Western Blot (WB). GTX101895

GTX101895 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with F4/80 antibody [C2C3], C-term (GTX101895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169