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F4/80 antibody [CI:A3-1] (Low endotoxin, azide free)

Anti-F4/80 antibody [CI:A3-1] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX41205

Cat. No. GTX41205

Reference 42

Host

 Rat

Clonality

 Monoclonal

Clone Name

 CI:A3-1

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC (Resin sections), IHC, Neutralizing/Inhibition, RIA

Reactivity

 Human, Mouse
Package
500 μg ($829)
See all F4/80 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 1/100-1/200
IP Assay dependent
IHC (Resin sections) Assay dependent
IHC Assay dependent
Neutralizing/Inhibition Assay dependent
RIA Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Neutralizing/Inhibition
Anti-F4/80 mAb specifically inhibits IFN-g production in HKL-stimulated SCID WSC cultures (Warschkau & Kiderlen, 1999).
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Product Note

This antibody recognizes murine F4/80 antigen which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.

Purification

Protein G purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

RRID

AB_11178061

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adhesion G protein-coupled receptor E1 , DD7A5-7 , EGF-TM7 , Emr1 , F4/80 , Gpf480 , Ly71 , TM7LN3

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-F4/80 antibody [CI:A3-1] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX41205

GTX41205 IHC-Fr Image

IHC-Fr analysis of mouse spleen tissue using GTX41205 F4/80 antibody at 1/100

REFERENCE

Clone Reference

Wang H et al. J Cardiovasc Pharmacol 2022; 80 (4):609-615 Sestrin2 Is Increased in Calcific Aortic Disease and Inhibits Osteoblastic Differentiation in Valvular Interstitial Cells via the Nuclear Factor E2-related Factor 2 Pathway.
Al Haq AT et al. Cell Death Dis 2022; 13 (1):49 Targeting prooxidant MnSOD effect inhibits triple-negative breast cancer (TNBC) progression and M2 macrophage functions under the oncogenic stress.
Chen HH et al. iScience 2021; 24 (9):103086 DDX3 modulates the tumor microenvironment via its role in endoplasmic reticulum-associated translation.
Chiang MT et al. Arterioscler Thromb Vasc Biol 2021; 41 (1):331-345 Gal-1 (Galectin-1) Upregulation Contributes to Abdominal Aortic Aneurysm Progression by Enhancing Vascular Inflammation.
Spiers JG et al. Biochimie 2021; 180:30-42 Repeated acute stress modulates hepatic inflammation and markers of macrophage polarisation in the rat.
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REVIEW

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Application Reference
Heng-Hsiung Wu et al. J Exp Med 2015; 212 (3):333-49 Targeting IL-17B-IL-17RB signaling with an anti-IL-17RB antibody blocks pancreatic cancer metastasis by silencing multiple chemokines.
Application : ICC/IF
Reactivity : Human
Bian Z et al. J Lipid Res 2013; 54 (1):44-54 CCN1 expression in hepatocytes contributes to macrophage infiltration in nonalcoholic fatty liver disease in mice.
Application : IHC-P
Reactivity : Mouse
Prins PA et al. Cell Physiol Biochem 2012; 29 (43102):121-30 Benzo[a]pyrene potentiates the pathogenesis of abdominal aortic aneurysms in apolipoprotein E knockout mice.
Application : IHC
Li CH et al. Toxicol Sci 2012; 126 (1):162-72 Zinc oxide nanoparticles-induced intercellular adhesion molecule 1 expression requires Rac1/Cdc42, mixed lineage kinase 3, and c-Jun N-terminal kinase activation in endothelial cells.
Application : IHC-P
Doyle MJ et al. J Cell Biol 2011; 195 (1):147-63 Abcg2 labels multiple cell types in skeletal muscle and participates in muscle regeneration.
Application : IHC
Reactivity : Mouse
Sampson UK et al. J Ultrasound Med 2011; 30 (6):773-84 Quantitative estimates of the variability of in vivo sonographic measurements of the mouse aorta for studies of abdominal aortic aneurysms and related arterial diseases.
Application : IHC
Reactivity : Mouse
Chung ES et al. Am J Pathol 2009; 175 (5):1984-92 Contribution of macrophages to angiogenesis induced by vascular endothelial growth factor receptor-3-specific ligands.
Application : IHC
Reactivity : Mouse
Clone Reference
Wang H et al. J Cardiovasc Pharmacol 2022; 80 (4):609-615 Sestrin2 Is Increased in Calcific Aortic Disease and Inhibits Osteoblastic Differentiation in Valvular Interstitial Cells via the Nuclear Factor E2-related Factor 2 Pathway.
Al Haq AT et al. Cell Death Dis 2022; 13 (1):49 Targeting prooxidant MnSOD effect inhibits triple-negative breast cancer (TNBC) progression and M2 macrophage functions under the oncogenic stress.
Yaw-Wen Hsu et al. J Biomed Sci 2021; 28 (1):5 Siglec-E retards atherosclerosis by inhibiting CD36-mediated foam cell formation.
Iman M Ahmad et al. Cancers (Basel) 2021; 13 (9)
Jung S et al. Antioxidants (Basel) 2021; 10 (9) Mulberry (Morus alba L.) Fruit Extract Ameliorates Inflammation via Regulating MicroRNA-21/132/143 Expression and Increases the Skeletal Muscle Mitochondrial Content and AMPK/SIRT Activities.
Li J et al. Ann Transl Med 2021; 9 (20):1534 Lupeol reduces M1 macrophage polarization to attenuate immunologic dissonance and fatty acid deposition in rats with diet-induced metabolic syndrome.
Hu JL et al. Transplant Proc 2021; 53 (7):2397-2406 Effects of Administration Route of Adipose-Derived Stem Cells on the Survival of Allogeneic Skin Grafts in Mice.
Chen HH et al. iScience 2021; 24 (9):103086 DDX3 modulates the tumor microenvironment via its role in endoplasmic reticulum-associated translation.
Chiang MT et al. Arterioscler Thromb Vasc Biol 2021; 41 (1):331-345 Gal-1 (Galectin-1) Upregulation Contributes to Abdominal Aortic Aneurysm Progression by Enhancing Vascular Inflammation.
Spiers JG et al. Biochimie 2021; 180:30-42 Repeated acute stress modulates hepatic inflammation and markers of macrophage polarisation in the rat.
Lin CY et al. PLoS One 2020; 15 (10):e0241053 Utilizing methylglyoxal and D-lactate in urine to evaluate saikosaponin C treatment in mice with accelerated nephrotoxic serum nephritis.
Duman M et al. Nat Commun 2020; 11 (1):3420 EEF1A1 deacetylation enables transcriptional activation of remyelination.
Chan YL et al. Cancers (Basel) 2020; 12 (7) TIAM2S Mediates Serotonin Homeostasis and Provokes a Pro-Inflammatory Immune Microenvironment Permissive for Colorectal Tumorigenesis.
Hall BM et al. Aging Cell 2020; 19 (10):e13219 Immune checkpoint protein VSIG4 as a biomarker of aging in murine adipose tissue.
Dufek B et al. PLoS One 2020; 15 (8):e0237907 RNA-seq analysis of gene expression profiles in isolated stria vascularis from wild-type and Alport mice reveals key pathways underling Alport strial pathogenesis.
Arturo A Wilkins-Rodr?guez et al. Infect Immun 2020; 88 (7) Differential Regulation of l-Arginine Metabolism through Arginase 1 during Infection with Leishmania mexicana Isolates Obtained from Patients with Localized and Diffuse Cutaneous Leishmaniasis.
Hirano Y et al. FEBS Open Bio 2020; 10 (5):894-903 Interleukin-36α as a Potential Biomarker for Renal Tubular Damage Induced by Dietary Phosphate Load.
Wu HM et al. J Gastroenterol Hepatol 2020; (Epub) Regulation of lipid-induced macrophage polarization through modulating peroxisome proliferator-activated receptor-gamma activity affects hepatic lipid metabolism via a Toll-like receptor 4/NF-庥B signaling pathway.
Chen LM et al. Cancers (Basel) 2020; 12 (2) Oligo-Fucoidan Prevents M2 Macrophage Differentiation and HCT116 Tumor Progression.
Han X et al. Br J Pharmacol 2020; (Epub) Myeloid-specific Dopamine D 2 Receptor Signalling Controls Inflammation in Acute Pancreatitis via Inhibiting M1 Macrophage.
Wang T et al. Front Pharmacol 2020; 11:45 Alleviation of Synovial Inflammation of Juanbi-Tang on Collagen-Induced Arthritis and TNF-Tg Mice Model.
Rong Y et al. Hum Vaccin Immunother 2019; An Intranasally Administered Monoclonal Antibody Cocktail Abrogates Ricin Toxin-Induced Pulmonary Tissue Damage and Inflammation.
Mooney B et al. J Leukoc Biol 2019; Sensitivity of Kupffer cells and liver sinusoidal endothelial cells to ricin toxin and ricin toxin-Ab complexes.
Van Raemdonck K et al. Cell Mol Life Sci 2019; CCL21/CCR7 signaling in macrophages promotes joint inflammation and Th17-mediated osteoclast formation in rheumatoid arthritis.
Vaqui? A et al. Cell Rep 2019; 27 (11):3152-3166.e7 Injured Axons Instruct Schwann Cells to Build Constricting Actin Spheres to Accelerate Axonal Disintegration.
Li B et al. Int Immunopharmacol 2018; 57:147-156 Substance P-regulated leukotriene B4 production promotes acute pancreatitis-associated lung injury through neutrophil reverse migration.
O'Brien MJ et al. Arthritis Res Ther 2018; 20 (1):31 A unique role for galectin-9 in angiogenesis and inflammatory arthritis.
Lee SW et al. J Evid Based Complementary Altern Med 2017; (Epub) euphorbia kansui attenuates * resistance in obese human subjects and high fat diet-induced obese mice.
Lai HY et al. Cardiovasc Res 2017; (Epub) CCAAT/enhancer-binding protein delta promotes intracellular lipid accumulation in * macrophages of vascular lesions.
Han X et al. Br J Pharmacol 2017; 174 (24):4751-4770 Dopamine D2 receptor signalling controls inflammation in acute pancreatitis via a PP2A-dependent Akt/NF-κB signalling pathway.
Luo W et al. Sci Rep 2017; 7:44612 Effect of modulation of PPAR-γ activity on Kupffer cells M1/M2 polarization in the development of non-alcoholic fatty liver disease.
Lin YL et al. Proc Natl Acad Sci U S A 2016; (Epub) in vivo amelioration of endogenous antitumor autoantibodies via low-dose p4n through the lta4h/* a/baff pathway.
Fang S et al. Neuromolecular Med 2016; Vorinostat Modulates the Imbalance of T Cell Subsets, Suppresses Macrophage Activity, and Ameliorates Experimental Autoimmune Uveoretinitis.
Liao CC et al. J Virol 2014; Immunodeficient Mouse Models with Different Disease Profiles by in vivo Infection with the Same Clinical Isolate of Enterovirus 71.
Zhang Y et al. Vasc Health Risk Manag 2008; 4 (5):1095-102 The development of abdominal aortic aneurysms in mice is enhanced by benzo(a)pyrene.
SDS
PBS.pdf
Package List Price ($)
$ 829
Custom Conjugations