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F4/80 antibody [CI:A3-1] (Low endotoxin, azide free)

Anti-F4/80 antibody [CI:A3-1] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX41205

Cat No. GTX41205

Host

Rat

Clonality

Monoclonal

Clone Name

CI:A3-1

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC (Resin sections), IHC, Neutralizing/Inhibition, RIA

Reactivity

Human, Mouse
Package
500 μg ($739)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 1/100-1/200
IP Assay dependent
IHC (Resin sections) Assay dependent
IHC Assay dependent
Neutralizing/Inhibition Assay dependent
RIA Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Neutralizing/Inhibition
Anti-F4/80 mAb specifically inhibits IFN-g production in HKL-stimulated SCID WSC cultures (Warschkau & Kiderlen, 1999).

Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Product Note

This antibody recognizes murine F4/80 antigen which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_11178061

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

adhesion G protein-coupled receptor E1 , DD7A5-7 , EGF-TM7 , Emr1 , F4/80 , Gpf480 , Ly71 , TM7LN3

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-F4/80 antibody [CI:A3-1] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX41205

GTX41205 IHC-Fr Image

IHC-Fr analysis of mouse spleen tissue using GTX41205 F4/80 antibody at 1/100

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf