GeneTex
United States (US)

F4/80 antibody [CI:A3-1]

GTX26640_3635_IHC-Fr_18121410_645.jpg

Cat No. GTX26640

Host Rat
Clonality Monoclonal
Clone Name CI:A3-1
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, EM, IHC-R, IHC, RIA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 1/50-1/100
IP Assay dependent
EM Assay dependent
IHC-R Assay dependent
IHC Assay dependent
RIA Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes murine F4/80 antigen which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Adhesion G Protein-Coupled Receptor E1,Dd7A5-7,Egf-Tm7,Emr1,F4/80,Gpf480,Ly71,Tm7Ln3,Adgre1

Database

Research Area

DATA IMAGES
GTX26640_3635_IHC-Fr_18121410_645.jpg

GTX26640 IHC-Fr Image

IHC-Fr analysis of mouse spleen tissue using GTX26640 F4/80 antibody [CI:A3-1].

REFERENCE
Application Reference
Package List Price ($)
$ 359