F4/80 antibody [HL5468]

F4/80 antibody [HL5468] detects F4/80 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
F4/80 stained by F4/80 antibody [HL5468] (GTX646110) diluted at 1:5000.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

F4/80 antibody [HL5468] detects F4/80 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
Green: F4/80 stained by F4/80 antibody [HL5468] (GTX646110) diluted at 1:5000 and highly cited rat monoclonal antibody diluted at 1:5000.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Unboiled various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with F4/80 antibody [HL5468] (GTX646110) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

F4/80 antibody [HL5468] detects F4/80 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: F4/80 stained by F4/80 antibody [HL5468] (GTX646110) diluted at 1:500.
Blue: Fluoroshield™ with DAPI (GTX30920).

F4/80 antibody [HL5468] detects F4/80 protein by immunofluorescent analysis.
Sample: Raw264.7 cells were fixed in ice-cold MeOH for 5 min and treated with Fc blocker after blocking step.
Green: F4/80 stained by F4/80 antibody [HL5468] (GTX646110) diluted at 1:500.
Blue: Fluoroshield™ with DAPI (GTX30920).

F4/80 antibody [HL5468] (GTX646110) detects Adgre1 protein by flow cytometry analysis.
Sample: Raw264.7 were blocked with an FcR blocking reagent for 10 minutes and fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with staning buffer.
The cells were following stained with F4/80 antibody [HL5468] (GTX646110) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) control at 4ºC for 1 hour.

F4/80 antibody [HL5468] detects F4/80 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
F4/80 stained by F4/80 antibody [HL5468] (GTX646110) diluted at 1:5000.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

F4/80 antibody [HL5468] detects F4/80 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebral cortex.
F4/80 stained by F4/80 antibody [HL5468] (GTX646110) diluted at 1:5000.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Unboiled various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with F4/80 antibody [HL5468] (GTX646110) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with F4/80 antibody [HL5468] (GTX646110) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with F4/80 antibody [HL5468] (GTX646110) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with F4/80 antibody [HL5468] (GTX646110) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.