APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Sheep, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human FABP4. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
fatty acid binding protein 4 , A-FABP , AFABP , ALBP , HEL-S-104 , aP2
Cellular Localization
Cytoplasm,Nucleus
Background
FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX636995 ICC/IF Image
FABP4 antibody [HL1521] detects FABP4 protein at cytoplasm by immunofluorescent analysis.Sample: RT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FABP4 stained by FABP4 antibody [HL1521] (GTX636995) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636995 IHC-P Image
FABP4 antibody [HL1521] detects FABP4 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brown adipocyte. FABP4 stained by FABP4 antibody [HL1521] (GTX636995) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636995 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [HL1521] (GTX636995) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636995 IHC-P Image
FABP4 antibody [HL1521] detects FABP4 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse white adipocyte. FABP4 stained by FABP4 antibody [HL1521] (GTX636995) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636995 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [HL1521] (GTX636995) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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REFERENCE
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REVIEW
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