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FABP4 antibody [HL1521]

Anti-FABP4 antibody [HL1521] used in Western Blot (WB). GTX636995
Anti-FABP4 antibody [HL1521] used in IHC (Paraffin sections) (IHC-P). GTX636995
Anti-FABP4 antibody [HL1521] used in Western Blot (WB). GTX636995
Anti-FABP4 antibody [HL1521] used in IHC (Paraffin sections) (IHC-P). GTX636995
Anti-FABP4 antibody [HL1521] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636995
Anti-FABP4 antibody [HL1521] used in Western Blot (WB). GTX636995

Cat. No. GTX636995

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1521

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human FABP4. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fatty acid binding protein 4 , A-FABP , AFABP , ALBP , HEL-S-104 , aP2

Cellular Localization

Cytoplasm,Nucleus

Background

FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FABP4 antibody [HL1521] used in Western Blot (WB). GTX636995

GTX636995 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [HL1521] (GTX636995) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-FABP4 antibody [HL1521] used in IHC (Paraffin sections) (IHC-P). GTX636995

GTX636995 IHC-P Image

FABP4 antibody [HL1521] detects FABP4 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
FABP4 stained by FABP4 antibody [HL1521] (GTX636995) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FABP4 antibody [HL1521] used in Western Blot (WB). GTX636995

GTX636995 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [HL1521] (GTX636995) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FABP4 antibody [HL1521] used in IHC (Paraffin sections) (IHC-P). GTX636995

GTX636995 IHC-P Image

FABP4 antibody [HL1521] detects FABP4 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse white adipocyte.
FABP4 stained by FABP4 antibody [HL1521] (GTX636995) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FABP4 antibody [HL1521] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636995

GTX636995 ICC/IF Image

FABP4 antibody [HL1521] detects FABP4 protein at cytoplasm by immunofluorescent analysis.
Sample: RT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FABP4 stained by FABP4 antibody [HL1521] (GTX636995) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-FABP4 antibody [HL1521] used in Western Blot (WB). GTX636995

GTX636995 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [HL1521] (GTX636995) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

FABP4 antibody [HL1521] Cat. No. GTX636995

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 28-Dec-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : A2058 cells
Amount used : 10μg
Blocking : 5% non-fat milk in TBST, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, 15Hr
Package List Price ($)
$ 399
$ 169
Custom Conjugations