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FABP4 antibody [N3C3]

Orthogonal
Anti-FABP4 antibody [N3C3] used in Western Blot (WB). GTX116036
Anti-FABP4 antibody [N3C3] used in Western Blot (WB). GTX116036
Anti-FABP4 antibody [N3C3] used in Western Blot (WB). GTX116036
Anti-FABP4 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116036
Anti-FABP4 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116036
Anti-FABP4 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116036

Cat. No. GTX116036

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all FABP4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FABP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036905

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fatty acid binding protein 4 , A-FABP , AFABP , ALBP , HEL-S-104 , aP2

Background

FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FABP4 antibody [N3C3] used in Western Blot (WB). GTX116036

GTX116036 WB Image

Orthogonal

Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [N3C3] (GTX116036) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

Anti-FABP4 antibody [N3C3] used in Western Blot (WB). GTX116036

GTX116036 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [N3C3] (GTX116036) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FABP4 antibody [N3C3] used in Western Blot (WB). GTX116036

GTX116036 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [N3C3] (GTX116036) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FABP4 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116036

GTX116036 IHC-P Image

FABP4 antibody [N3C3] detects FABP4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
FABP4 stained by FABP4 antibody [N3C3] (GTX116036) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FABP4 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116036

GTX116036 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FABP4(GTX116036) antibody at 1:200 dilution.

Anti-FABP4 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116036

GTX116036 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using FABP4 (adipocyte)(GTX116036) antibody at 1:200 dilution.

REFERENCE

Application Reference

Tsai YT et al. Nutrients 2020; 12 (3) Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice.
Saha PK et al. Am J Physiol Endocrinol Metab 2020; 319 (4) : E667-E677 miR-30a targets gene networks that promote browning of human and mouse adipocytes.
Application : WB
Reactivity : Human
Muszy?ska B et al. Biol Trace Elem Res 2019; (Epub) Lentinula edodes as a Source of Bioelements Released into Artificial Digestive Juices and Potential Anti-inflammatory Material.
Application : WB
Reactivity : Mouse
Kobayashi K et al. Traditional & Kampo Medicine 2017; (Epub) Anti-obesity and anti-inflammatory effects of peroxisome proliferator activated receptor-γ induced by bofutsushosan powder versus extract.
Application : ELISA
Reactivity : Mouse
Chen P et al. JBN 2017; 13 : 324-336 Micron/Submicron Hybrid Topography of Titanium Surfaces Influences Adhesion and Differentiation Behaviors of the Mesenchymal Stem Cells.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169