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FABP4 antibody [N3C3]

Anti-FABP4 antibody [N3C3] used in Western Blot (WB). GTX116036
Anti-FABP4 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116036
Anti-FABP4 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116036
Anti-FABP4 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116036
Anti-FABP4 antibody [N3C3] used in Western Blot (WB). GTX116036

Cat No. GTX116036

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FABP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036905

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fatty acid binding protein 4 , A-FABP , AFABP , ALBP , HEL-S-104 , aP2

Background

FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FABP4 antibody [N3C3] used in Western Blot (WB). GTX116036

GTX116036 WB Image

Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [N3C3] (GTX116036) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

Anti-FABP4 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116036

GTX116036 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using FABP4 (adipocyte)(GTX116036) antibody at 1:200 dilution.

Anti-FABP4 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116036

GTX116036 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FABP4(GTX116036) antibody at 1:200 dilution.

Anti-FABP4 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX116036

GTX116036 IHC-P Image

FABP4 antibody [N3C3] detects FABP4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
FABP4 stained by FABP4 antibody [N3C3] (GTX116036) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FABP4 antibody [N3C3] used in Western Blot (WB). GTX116036

GTX116036 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [N3C3] (GTX116036) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169