APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NT2D1 , IMR32 , mouse brain , mouse fetal brain , rat brain
Predict Reactivity
Bovine, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human FABP7 Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11166611
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fatty acid binding protein 7 , B-FABP , BLBP , FABPB , MRG
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a brain fatty acid binding protein. Fatty acid binding proteins (FABPs) are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs are thought to play roles in fatty acid uptake, transport, and metabolism. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX121467 IHC-Fr Image
BLBP antibody [N1C3] detects BLBP protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: BLBP protein stained by BLBP antibody [N1C3] (GTX121467) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GTX121467 ICC/IF Image
FABP7 antibody [N1C3] detects FABP7 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FABP7 stained by FABP7 antibody [N1C3] (GTX121467) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX121467 WB Image
Sample (30 μg of whole cell lysate) A: whole zebrafish B: Zebrafish brain 15% SDS PAGE GTX121467 diluted at 1:1000
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GTX121467 WB Image
Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 15% SDS PAGE GTX121467 diluted at 1:1000
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GTX121467 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with BLBP antibody [N1C3] (GTX121467) diluted at 1:1000.
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REFERENCE
There are currently no references for FABP7 antibody [N1C3] (GTX121467). Be the first to share your publications with this product.
REVIEW
FABP7 antibody [N1C3]
Cat. No. GTX121467
Rating
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( Average 5 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 14-Feb-2023
Application Tested :
WB
Sample Species :
Ms
Sample :
Mouse brain tissue lysate
Amount used :
10μg
Blocking :
5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody :
1:500, 16°C, 25Hr