APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
Predict Reactivity
Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.28 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FACL4. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888336
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
acyl-CoA synthetase long chain family member 4 , ACS4 , FACL4 , LACS4 , MRX63 , MRX68
Cellular Localization
Mitochondrion outer membrane; Single-pass type III membrane protein , Peroxisome membrane; Single-pass type III membrane protein , Microsome membrane; Single-pass type III membrane protein , Endoplasmic reticulum membrane; Single-pass type III membrane pr
Background
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX633365 IHC-P Image
FACL4 antibody [GT186] detects FACL4 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.FACL4 stained by FACL4 antibody [GT186] (GTX633365) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX633365 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [GT186] (GTX633365) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX633365 ICC/IF Image
FACL4 antibody [GT186] detects FACL4 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: FACL4 stained by FACL4 antibody [GT186] (GTX633365) diluted at 1:100.Blue: Hoechst 33342 staining.
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GTX633365 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [GT186] (GTX633365) diluted at 1:500.
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GTX633365 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [GT186] (GTX633365) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for FACL4 antibody [GT186] (GTX633365). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FACL4 antibody [GT186] (GTX633365). Be the first to share your experience with this product.