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FACL4 antibody [GT186]

Anti-FACL4 antibody [GT186] used in Western Blot (WB). GTX633365
Anti-FACL4 antibody [GT186] used in IHC (Paraffin sections) (IHC-P). GTX633365
Anti-FACL4 antibody [GT186] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633365

Cat No. GTX633365

Host

Mouse

Clonality

Monoclonal

Clone Name

GT186

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human FACL4. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

acyl-CoA synthetase long chain family member 4 , ACS4 , FACL4 , LACS4 , MRX63 , MRX68

Cellular Localization

Mitochondrion outer membrane; Single-pass type III membrane protein , Peroxisome membrane; Single-pass type III membrane protein , Microsome membrane; Single-pass type III membrane protein , Endoplasmic reticulum membrane; Single-pass type III membrane pr

Background

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FACL4 antibody [GT186] used in Western Blot (WB). GTX633365

GTX633365 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [GT186] (GTX633365) diluted at 1:500.

Anti-FACL4 antibody [GT186] used in IHC (Paraffin sections) (IHC-P). GTX633365

GTX633365 IHC-P Image

FACL4 antibody [GT186] detects FACL4 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
FACL4 stained by FACL4 antibody [GT186] (GTX633365) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FACL4 antibody [GT186] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633365

GTX633365 ICC/IF Image

FACL4 antibody [GT186] detects FACL4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: FACL4 stained by FACL4 antibody [GT186] (GTX633365) diluted at 1:100.
Blue: Hoechst 33342 staining.

REFERENCE

There are currently no references for FACL4 antibody [GT186] (GTX633365). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for FACL4 antibody [GT186] (GTX633365). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169