GeneTex

  • Country / Location Selection

United States (US)

FACL4 antibody [HL229]

Recombinant antibody KO/KD Orthogonal
Anti-FACL4 antibody [HL229] used in Western Blot (WB). GTX635616
Anti-FACL4 antibody [HL229] used in Western Blot (WB). GTX635616
Anti-FACL4 antibody [HL229] used in Western Blot (WB). GTX635616
Anti-FACL4 antibody [HL229] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635616
Anti-FACL4 antibody [HL229] used in IHC (Paraffin sections) (IHC-P). GTX635616
Anti-FACL4 antibody [HL229] used in Western Blot (WB). GTX635616
Anti-FACL4 antibody [HL229] used in Western Blot (WB). GTX635616
Anti-FACL4 antibody [HL229] used in IHC (Paraffin sections) (IHC-P). GTX635616

Cat. No. GTX635616

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL229

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all FACL4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FACL4. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888541

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acyl-CoA synthetase long chain family member 4 , ACS4 , FACL4 , LACS4 , MRX63 , MRX68

Cellular Localization

Mitochondrion outer membrane,Endoplasmic reticulum membrane

Background

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-FACL4 antibody [HL229] used in Western Blot (WB). GTX635616

GTX635616 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (GTX635616) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FACL4 antibody [HL229] used in Western Blot (WB). GTX635616

GTX635616 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (GTX635616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FACL4 antibody [HL229] used in Western Blot (WB). GTX635616

GTX635616 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (GTX635616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FACL4 antibody [HL229] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635616

GTX635616 ICC/IF Image

FACL4 antibody [HL229] detects FACL4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FACL4 stained by FACL4 antibody [HL229] (GTX635616) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-FACL4 antibody [HL229] used in IHC (Paraffin sections) (IHC-P). GTX635616

GTX635616 IHC-P Image

FACL4 antibody [HL229] detects FACL4 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
FACL4 stained by FACL4 antibody [HL229] (GTX635616) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FACL4 antibody [HL229] used in Western Blot (WB). GTX635616

GTX635616 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (GTX635616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-FACL4 antibody [HL229] used in Western Blot (WB). GTX635616

GTX635616 WB Image

Drosophila tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (GTX635616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-FACL4 antibody [HL229] used in IHC (Paraffin sections) (IHC-P). GTX635616

GTX635616 IHC-P Image

FACL4 antibody [HL44] detects FACL4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
FACL4 stained by FACL4 antibody [HL44] (GTX635616) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for FACL4 antibody [HL229] (GTX635616). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for FACL4 antibody [HL229] (GTX635616). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations