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FACL4 antibody [N1N3]

KO/KD Orthogonal
Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399
Anti-FACL4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114399
Anti-FACL4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114399
Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399
Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399
Anti-FACL4 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114399
Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399
Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399
Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399

Cat. No. GTX114399

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all FACL4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

HeLa , Raw264.7 , PC-12 , Rat-2 , MCF-7 , MDA-MB-231

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FACL4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619561

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acyl-CoA synthetase long chain family member 4 , ACS4 , FACL4 , LACS4 , MRX63 , MRX68

Cellular Localization

Mitochondrion outer membrane; Single-pass type III membrane protein , Peroxisome membrane; Single-pass type III membrane protein , Microsome membrane; Single-pass type III membrane protein , Endoplasmic reticulum membrane; Single-pass type III membrane pr

Background

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399

GTX114399 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (GTX114399) diluted at 1:1000.

Anti-FACL4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114399

GTX114399 IHC-P Image

FACL4 antibody [N1N3] detects FACL4 protein at cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
FACL4 antibody [N1N3] (GTX114399) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FACL4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX114399

GTX114399 IHC-P Image

FACL4 antibody [N1N3] detects FACL4 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
FACL4 antibody [N1N3] (GTX114399) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399

GTX114399 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
FACL4 antibody
GTX114399 diluted at 1:1000

Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399

GTX114399 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (GTX114399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FACL4 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114399

GTX114399 ICC/IF Image

FACL4 antibody [N1N3] detects FACL4 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FACL4 protein stained by FACL4 antibody [N1N3] (GTX114399) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399

GTX114399 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (GTX114399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399

GTX114399 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (GTX114399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FACL4 antibody [N1N3] used in Western Blot (WB). GTX114399

GTX114399 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (GTX114399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Tsai YT et al. Nutrients 2020; 12 (3) Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169