GeneTex
United States (US)

FADD antibody

KO/KD Orthogonal
GTX100612_39918_20170922_WB_KO_watermark_19011506_435.jpg
GTX100612_39918_20170922_WB_Cisplatin_competitor_watermark_19011506_275.jpg
GTX100612_39918_20181207_IHC-P_19011519_315.jpg

Cat No. GTX100612

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

HeLa , HeLa (30 μM Cisplatin treatment for 24 hr)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of Human FADD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fas Associated Via Death Domain,Gig3,Mort1,Fadd

Background

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100612_39918_20170922_WB_KO_watermark_19011506_435.jpg

GTX100612 WB Image

KO/KD

Wild-type (WT) and FADD knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with FADD antibody [N1C3] (GTX100612) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX100612_39918_20170922_WB_Cisplatin_competitor_watermark_19011506_275.jpg

GTX100612 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with FADD antibody [N1C3] (GTX100612) diluted at 1:500 and competitor's antibody (sc-6035) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX100612_39918_20181207_IHC-P_19011519_315.jpg

GTX100612 IHC-P Image

FADD antibody detects FADD protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
FADD stained by FADD antibody (GTX100612) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169