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FADS1 antibody

Anti-FADS1 antibody used in Western Blot (WB). GTX114528
Anti-FADS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114528
Anti-FADS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114528

Cat. No. GTX114528

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FADS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human FADS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166493

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fatty acid desaturase 1 , D5D , FADS6 , FADSD5 , LLCDL1 , TU12

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FADS1 antibody used in Western Blot (WB). GTX114528

GTX114528 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX114528 diluted at 1:1000

Anti-FADS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114528

GTX114528 ICC/IF Image

FADS1 antibody detects FADS1 protein at mitochondria by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: FADS1 protein stained by FADS1 antibody (GTX114528) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-FADS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114528

GTX114528 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using FADS1(GTX114528) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Lee JY et al. Proc Natl Acad Sci U S A 2020; 117 (51) : 32433-32442 Polyunsaturated fatty acid biosynthesis pathway determines ferroptosis sensitivity in gastric cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169