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FAH antibody [HL1970]

Recombinant antibody KO/KD Orthogonal
Anti-FAH antibody [HL1970] used in Western Blot (WB). GTX637863
Anti-FAH antibody [HL1970] used in Western Blot (WB). GTX637863
Anti-FAH antibody [HL1970] used in Western Blot (WB). GTX637863
Anti-FAH antibody [HL1970] used in IHC (Paraffin sections) (IHC-P). GTX637863
Anti-FAH antibody [HL1970] used in Western Blot (WB). GTX637863

Cat. No. GTX637863

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1970

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all FAH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Predict Reactivity

Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human FAH

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fumarylacetoacetate hydrolase

Background

This gene encodes the last enzyme in the tyrosine catabolism pathway. FAH deficiency is associated with Type 1 hereditary tyrosinemia (HT). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FAH antibody [HL1970] used in Western Blot (WB). GTX637863

GTX637863 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FAH antibody [HL1970] (GTX637863) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FAH antibody [HL1970] used in Western Blot (WB). GTX637863

GTX637863 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FAH antibody [HL1970] (GTX637863) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FAH antibody [HL1970] used in Western Blot (WB). GTX637863

GTX637863 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with FAH antibody [HL1970] (GTX637863) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FAH antibody [HL1970] used in IHC (Paraffin sections) (IHC-P). GTX637863

GTX637863 IHC-P Image

FAH antibody [HL1970] detects FAH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
FAH stained by FAH antibody [HL1970] (GTX637863) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FAH antibody [HL1970] used in Western Blot (WB). GTX637863

GTX637863 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FAH antibody [HL1970] (GTX637863) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations