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FAH antibody

Orthogonal
Anti-FAH antibody used in IHC (Paraffin sections) (IHC-P). GTX114400
Anti-FAH antibody used in IHC (Paraffin sections) (IHC-P). GTX114400
Anti-FAH antibody used in Western Blot (WB). GTX114400
Anti-FAH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114400
Anti-FAH antibody used in Western Blot (WB). GTX114400
Anti-FAH antibody used in Western Blot (WB). GTX114400

Cat. No. GTX114400

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all FAH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

mouse kidney , mouse liver

Predict Reactivity

Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fumarylacetoacetate hydrolase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10622902

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fumarylacetoacetate hydrolase

Background

This gene encodes the last enzyme in the tyrosine catabolism pathway. FAH deficiency is associated with Type 1 hereditary tyrosinemia (HT). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FAH antibody used in IHC (Paraffin sections) (IHC-P). GTX114400

GTX114400 IHC-P Image

FAH antibody detects FAH protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
FAH stained by FAH antibody (GTX114400) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FAH antibody used in IHC (Paraffin sections) (IHC-P). GTX114400

GTX114400 IHC-P Image

FAH antibody detects FAH protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
FAH stained by FAH antibody (GTX114400) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FAH antibody used in Western Blot (WB). GTX114400

GTX114400 WB Image

Fumarylacetoacetate hydrolase antibody detects FAH protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
10 % SDS-PAGE
Fumarylacetoacetate hydrolase antibody (GTX114400) dilution: 1:1000

Anti-FAH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114400

GTX114400 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Fumarylacetoacetase(GTX114400) antibody at 1:200 dilution.

Anti-FAH antibody used in Western Blot (WB). GTX114400

GTX114400 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FAH antibody (GTX114400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-FAH antibody used in Western Blot (WB). GTX114400

GTX114400 WB Image

Fumarylacetoacetate hydrolase antibody detects FAH protein by Western blot analysis.
A. 50 μg mouse kidney lysate/extract
10 % SDS-PAGE
Fumarylacetoacetate hydrolase antibody (GTX114400) dilution: 1:1000

REFERENCE

Application Reference

Guo R et al. Nat Commun 2022; 13 (1) : 7345 IL6 supports long-term expansion of hepatocytes in vitro.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169