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FAK antibody [10H7]

Anti-FAK antibody [10H7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82780
Anti-FAK antibody [10H7] used in Western Blot (WB). GTX82780
Anti-FAK antibody [10H7] used in IHC (Paraffin sections) (IHC-P). GTX82780

Cat. No. GTX82780

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10H7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all FAK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human FAK expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine kinase 2 , FADK , FAK , FAK1 , FRNK , PPP1R71 , p125FAK , pp125FAK

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-FAK antibody [10H7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82780

GTX82780 ICC/IF Image

ICC/IF analysis of B16 cells using GTX82780 FAK antibody [10H7].
Green : FAK
Blue: DRAQ5 fluorescent DNA dye

Anti-FAK antibody [10H7] used in Western Blot (WB). GTX82780

GTX82780 WB Image

WB analysis of A549 (1) and NIH3T3 (2) cell lysate using GTX82780 FAK antibody [10H7].

Anti-FAK antibody [10H7] used in IHC (Paraffin sections) (IHC-P). GTX82780

GTX82780 IHC-P Image

IHC-P analysis of cervices tumour using GTX82780 FAK antibody [10H7].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399