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FAK antibody [SR46-04]

Recombinant antibody
Anti-FAK antibody [SR46-04] used in Western Blot (WB). GTX01141
Anti-FAK antibody [SR46-04] used in Western Blot (WB). GTX01141
Anti-FAK antibody [SR46-04] used in IHC (Paraffin sections) (IHC-P). GTX01141
Anti-FAK antibody [SR46-04] used in IHC (Paraffin sections) (IHC-P). GTX01141
Anti-FAK antibody [SR46-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01141
Anti-FAK antibody [SR46-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01141
Anti-FAK antibody [SR46-04] used in Flow cytometry (FACS). GTX01141
Anti-FAK antibody [SR46-04] used in IHC (Paraffin sections) (IHC-P). GTX01141
Anti-FAK antibody [SR46-04] used in IHC (Paraffin sections) (IHC-P). GTX01141

Cat No. GTX01141

Host

Rabbit

Clonality

Monoclonal

Clone Name

SR46-04

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein tyrosine kinase 2 , FADK , FAK , FAK1 , FRNK , PPP1R71 , PTK2 , p125FAK , pp125FAK

Cellular Localization

Cell junction,Focal adhesion,Cell membrane,Cytoplasm,Cell cortex,Cytoskeleton,Microtubule organizing center,Centrosome,Nucleus

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]

Database

Research Area

DATA IMAGES

Anti-FAK antibody [SR46-04] used in Western Blot (WB). GTX01141

GTX01141 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK antibody [SR46-04] (GTX01141) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FAK antibody [SR46-04] used in Western Blot (WB). GTX01141

GTX01141 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK antibody [SR46-04] (GTX01141) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FAK antibody [SR46-04] used in IHC (Paraffin sections) (IHC-P). GTX01141

GTX01141 IHC-P Image

IHC-P analysis of huamn liver cancer tissue section using GTX01141 FAK antibody [SR46-04].

Anti-FAK antibody [SR46-04] used in IHC (Paraffin sections) (IHC-P). GTX01141

GTX01141 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX01141 FAK antibody [SR46-04].

Anti-FAK antibody [SR46-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01141

GTX01141 ICC/IF Image

ICC/IF analysis of PFA-fixed PANC-1 cells using GTX01141 FAK antibody [SR46-04].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-FAK antibody [SR46-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01141

GTX01141 ICC/IF Image

ICC/IF analysis of PFA-fixed SH-SY-5Y cells using GTX01141 FAK antibody [SR46-04].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-FAK antibody [SR46-04] used in Flow cytometry (FACS). GTX01141

GTX01141 FACS Image

FACS analysis of HeLa cells using GTX01141 FAK antibody [SR46-04].br>Blue : primary antibody
Red : unlabelled control
Dilution : 1:50

Anti-FAK antibody [SR46-04] used in IHC (Paraffin sections) (IHC-P). GTX01141

GTX01141 IHC-P Image

IHC-P analysis of mouse spleen tissue section using GTX01141 FAK antibody [SR46-04].

Anti-FAK antibody [SR46-04] used in IHC (Paraffin sections) (IHC-P). GTX01141

GTX01141 IHC-P Image

IHC-P analysis of human spleen tissue section using GTX01141 FAK antibody [SR46-04].

REFERENCE

There are currently no references for FAK antibody [SR46-04] (GTX01141). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for FAK antibody [SR46-04] (GTX01141). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf