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FAK antibody

Anti-FAK antibody used in Western Blot (WB). GTX100764
Anti-FAK antibody used in Western Blot (WB). GTX100764
Anti-FAK antibody used in Western Blot (WB). GTX100764
Anti-FAK antibody used in IHC (Paraffin sections) (IHC-P). GTX100764
Anti-FAK antibody used in IHC (Paraffin sections) (IHC-P). GTX100764
Anti-FAK antibody used in Western Blot (WB). GTX100764

Cat. No. GTX100764

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human FAK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619410

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine kinase 2 , FADK , FAK , FAK1 , FRNK , PPP1R71 , p125FAK , pp125FAK

Cellular Localization

Cell junction , focal adhesion , Cell membrane; Peripheral membrane protein; Cytoplasmic side

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FAK antibody used in Western Blot (WB). GTX100764

GTX100764 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (GTX100764) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-FAK antibody used in Western Blot (WB). GTX100764

GTX100764 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (GTX100764) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FAK antibody used in Western Blot (WB). GTX100764

GTX100764 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (GTX100764) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FAK antibody used in IHC (Paraffin sections) (IHC-P). GTX100764

GTX100764 IHC-P Image

FAK antibody detects FAK protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
FAK stained by FAK antibody (GTX100764) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FAK antibody used in IHC (Paraffin sections) (IHC-P). GTX100764

GTX100764 IHC-P Image

FAK antibody detects FAK protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
FAK stained by FAK antibody (GTX100764) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FAK antibody used in Western Blot (WB). GTX100764

GTX100764 WB Image

The data was published in the journal Evid Based Complement Alternat Med in 2012.PMID: 23320038

REFERENCE

REVIEW

FAK antibody Cat. No. GTX100764

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 21-Sep-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : ARPE-19 cells
Blocking : 3% BSA and 0.02% Triton-X100 in PBS, 25°C, 1Hr
Primary Antibody : 1:50, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169