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FAK (phospho Tyr397) antibody

Anti-FAK (phospho Tyr397) antibody used in Western Blot (WB). GTX24803
Anti-FAK (phospho Tyr397) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24803

Cat No. GTX24803

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, IP, ChIP assay

Reactivity

Human, Mouse, Rat, Zebrafish, Drosophila, Cat, Dog, Chicken, Amphibians, Rhesus Monkey, Xenopus, Fish
Package
50 μl ($259)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 397. The sequence is conserved in mouse, rat, chicken and frog.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380431

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein tyrosine kinase 2 , FADK , FAK , FAK1 , FRNK , PPP1R71 , p125FAK , pp125FAK

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]

Database

Research Area

DATA IMAGES

Anti-FAK (phospho Tyr397) antibody used in Western Blot (WB). GTX24803

GTX24803 WB Image

WB analysis of samples using GTX24803 FAK (phospho Tyr397) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphotyrosine containing peptide(Lane 3), the phosphopeptide corresponding to FAK (phospho Tyr402) , or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

Anti-FAK (phospho Tyr397) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24803

GTX24803 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX24803 FAK (phospho Tyr397) antibody.
Green : Primary antibody
Blue : cell nucleus
Fixation/Permeabilization : ice-cold 95% methanol

REFERENCE

REVIEW

FAK (phospho Tyr397) antibody Cat No. GTX24803

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2012.PMID: 23320038
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 27306323
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf