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FAK (phospho Tyr397) antibody

Orthogonal
Anti-FAK (phospho Tyr397) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24803
Anti-FAK (phospho Tyr397) antibody used in Western Blot (WB). GTX24803
Anti-FAK (phospho Tyr397) antibody used in Western Blot (WB). GTX24803
Anti-FAK (phospho Tyr397) antibody used in Western Blot (WB). GTX24803

Cat. No. GTX24803

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Zebrafish, Drosophila, Cat, Dog, Chicken, Amphibians, Rhesus Monkey, Xenopus, Fish
Package
50 μl ($399)
See all FAK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 50% Glycerol, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 397. The sequence is conserved in mouse, rat, chicken and frog.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380431

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine kinase 2 , FADK , FAK , FAK1 , FRNK , PPP1R71 , p125FAK , pp125FAK

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]

Database

Research Area

DATA IMAGES

Anti-FAK (phospho Tyr397) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24803

GTX24803 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX24803 FAK (phospho Tyr397) antibody.
Green : Primary antibody
Blue : cell nucleus
Fixation/Permeabilization : ice-cold 95% methanol

Anti-FAK (phospho Tyr397) antibody used in Western Blot (WB). GTX24803

GTX24803 WB Image

Orthogonal

WB analysis of samples using GTX24803 FAK (phospho Tyr397) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphotyrosine containing peptide(Lane 3), the phosphopeptide corresponding to FAK (phospho Tyr402) , or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

Anti-FAK (phospho Tyr397) antibody used in Western Blot (WB). GTX24803

GTX24803 WB Image

Orthogonal

The data was published in the journal Nat Commun in 2016.PMID: 27306323

Anti-FAK (phospho Tyr397) antibody used in Western Blot (WB). GTX24803

GTX24803 WB Image

Orthogonal

The data was published in the journal Evid Based Complement Alternat Med in 2012.PMID: 23320038

REFERENCE

Application Reference

Kuroda J et al. Front Cell Dev Biol 2020; 8 : 580520 The Physical Role of Mesenchymal Cells Driven by the Actin Cytoskeleton Is Essential for the Orientation of Collagen Fibrils in Zebrafish Fins.
Reactivity : Zebrafish
Terasaki M et al. Oncol Rep 2018; (Epub) Glycine and succinic acid are effective indicators of the suppression of epithelial-mesenchymal transition by fucoxanthinol in colorectal cancer stem-like cells.
Liu CC et al. Nat Commun 2016; 7 : 11798 Suspension survival mediated by PP2A-STAT3-Col XVII determines tumour initiation and metastasis in cancer stem cells.
Application : WB
Reactivity : Human
McGrail DJ et al. Sci Rep 2015; 5 : 9529 Alterations in ovarian cancer cell adhesion drive * resistance by increasing microtubule dynamics in a FAK-dependent manner.
Application : ICC/IF
Reactivity : Human
McAndrews KM et al. Sci Rep 2015; 5 : 16941 Mesenchymal Stem Cells Induce Directional Migration of Invasive Breast Cancer Cells through TGF-β.
Application : ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399